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Our Research


A major response of cells during pathogen infection is changes in gene expression, leading to changes in the proteins being produced in the cell. Proteins are essential biopolymers in all living organisms, playing roles as structural components of cells, enzymes, and immune response agents such as antibodies. Regulation of gene expression can occur at two levels: transcription (where mRNA is synthesised in the cell nucleus by the macromolecular machine RNA polymerase) and translation (where mRNA is decoded into proteins in the cell cytoplasm by the macromolecular machine known as the ribosome).

When cells are stressed, specific gene expression pathways are activated (e.g. cytokines as part of the innate immune system). However, so far, very few studies have systematically studied these changes in gene expression at the level of translation. The primary reason is due to technical difficulties with global monitoring of protein synthesis. Transcriptional regulation has been previously studied; however there is evidence that a significant amount of regulation also occurs at the translational level. This makes sense as direct modulation of protein synthesis provides a faster and more efficient response to pathogen infection, as it circumvents de novo mRNA transcription, processing and transport to the cell cytoplasm. Translational control is a highly dynamic process and global studies have only recently become possible with the advent of several high-throughput technologies, such as ribosome profiling - a high-throughput technique that allows capturing the location and abundance of all ribosomes on mRNAs, allowing precise global measurement of real-time protein synthesis. We aim towards understanding the complex interplay of host and pathogen gene regulation, and its ultimate effect on the host proteome and host response to biotic stress.

Dr Betty Chung

Principal Investigator



Group members:

Dr Sherine Thomas

Ms Arlia Rapaiee

Dr Filip Lastovka

Dr George Wood

Dr Li Yang

Mr Vebushan Sukumar

Dr Sam Crawshaw

Visiting scientists

Dr Mariya Lobanovska (UC Berkeley, Portnoy Lab)

Dr Alex Murphy (Department of Plant Sciences, Carr Lab)

Dr Jessica Bergman (Department of Plant Sciences, Carr Lab)

Dr Andrew Yep (Department of Plant Sciences, Carr Lab)