For enquires about any of these services, please contact Mike (msh57@cam.ac.uk).
Resin embedding electron microscopy
Routine embedding of cells of tissue for morphological electron microscopy studies.
Cryo immunogold electron microscopy
Cryosectioning of cells or tissues, followed by colloidal gold particle labelling of antigens (Tokuyasu method). Gives a higher immuno resolution versus confocal techniques.
Negative stain electron microscopy
Negative staining for bacteria, viruses, molecules.
Correlative Light and Electron Microscopy (CLEM)
A method to correlate light microscopy data with electron micrographs – allowing protein localisation and subcellular compartments to be identified.
Histology
Allows one to image cells in the context of a tissue, by cutting sections of tissue on a microtome. These sections can then be stained by Hematoxylin and eosin (H&E), staining the nucleus and cytosol in blue and pink respectively. Alternatively, perform immunohistochemistry on your tissue samples to detect specific antigens via fluorescence microscopy.
For other services, and specialist microscopy, please contact the Cambridge Advanced Imaging Centre (CAIC). https://caic.bio.cam.ac.uk
Specialist equipment including, Two-Photon, STED, Scanning Electron Microscopy.
