Instruments
Analysers:
- Aurora Spectral Analyser
- Attune NxT
- CytoFLEX S
- CytoFLEX LX
- LSR II
Cell sorters:
- Beckman Coulter MoFlo Astrios
- BD Aria III
- BD Aria IIu.
We welcome enquiries regarding possible uses.
The configurations of all instruments are available upon request or for registered users on PPMS.
Analysers
The analyzers are user-operated, but all users require at least one training session before being used independently. Training sessions should be requested on PPMS.
Cytek Aurora
The Cytek Aurora is a different flow cytometer that works by measuring the full spectral output excited by each laser and deconvoluting signals according to prerecorded fluorochrome characteristics. This means it is easier for you to run many different fluorescent probes, including ones previously too similar to use in combination (though they still need to be different spectrally to some degree). The Aurora has five lasers at 488nm (blue), 561nm (yellow-green), 633nm (red), 355nm (UV), and 405nm (violet). The Aurora also has a plate reader capable of running samples from 96-well plates.
More information about the spectral analysis
Attune NxT
The Attune NxT is equipped with four lasers (488nm (blue), 637nm (red), 405nm (violet), and 561nm (yellow-green)) and is capable of detecting 16 parameters (FSC, SSC, and 14 fluorescent parameters). Equipped with an autosampler compatible with many different plate formats, including 96-well, 384-well, and deep-well plates. The Attune is equipped with acoustic-assisted hydrodynamic focusing and can achieve sample-throughput rates up to 10 times faster than traditional cytometers. This means you can process samples more quickly and acquire sufficient events even from very dilute samples. In addition, it accommodates no-wash, no-lyse protocols, allowing you to run whole blood without lysis.
More information about acoustic focusing
CytoFLEX S
The CytoFLEX S is equipped with four lasers (488nm (blue), 640nm (red), 405nm (violet), and 561nm (yellow-green) and is capable of detecting 15 parameters (FSC, SSC, and 13 fluorescent parameters). It can run samples from 96-well plates. The CytoFLEX S is designed to be user-friendly, and users should be able to run it independently after a training session with a member of the facility staff.
More information about the Cytoflex S
CytoFLEX LX
The CytoFLEX LX is equipped with five lasers, 488nm (blue), 638nm (red), 405nm (violet), 561nm (yellow-green), and 35nm (UV), and is capable of detecting 21 parameters (FSC, SSC, and 19 fluorescent parameters). It can run samples from 96-well plates. The CytoFLEX LX is designed to be user-friendly, and users should be able to run it independently after a training session with a member of the facility staff.
More information about the CytoFlex LX
LSR II
The LSR II is equipped with four lasers (488nm (blue), 355nm (UV), 405nm (violet), and 633nm (red)) and is capable of detecting 19 parameters (FSC, SSC, and 17 fluorescent parameters). The LSR II is a user-friendly flow cytometer and runs BD FACSDiva, a software familiar to many users. Having a UV laser, it is the ideal instrument for running calcium flux experiments using Indo-1.
Cell sorters
MoFLo Astrios
The MoFlo Astrios has five lasers (355, 405, 488, 561 and 640nm) and a maximum of 18 fluorescent detectors and six scatter detectors. It also is contained within a Class II Biohazard cabinet. We welcome enquiries for sorting Class II material (subject to risk assessment) but also expect to sort typical class I material. It can sort cells in bulk or deposit any chosen number in multiwell trays of up to 384-wells or onto slides. The machine can sort six different populations simultaneously, maximising the use of your samples.
Aria III
The Aria III has four lasers (405, 488, 561 and 633nm), 16 fluorescent detectors, and two scatters. The Aria III is also contained within a class II biohazardous cabinet and can be used to sort CLASS I and II samples. The Aria can also sort cells in bulk (up to 4 populations simultaneously) or into multiwell trays or slides.
Aria Ilu
The Aria IIu has three lasers (405, 488 and 633nm), nine fluorescent detectors and two scatters. The Aria is not contained in a biohazard cabinet and can only be used with CLASS I samples. The Aria can also sort cells in bulk (up to 4 populations simultaneously) or into multiwell trays or slides.
Data Analysis
We also provide, free of charge, two stand-alone computer workstations for flow data analysis for facility users. Both PCs run FlowJo. Other available software: Kaluza, Summit, Attune NxT software, SpectroFlo, CytExpert.