Research
Our laboratory studies virus-cell interactions with a focus on the assembly and egress of viruses ane the wide variety of cellular pathways modified by viruses to establish infection and spread to new cells. We currently study three families of viral pathogens: herpesviruses, polyomaviruses and bunyaviruses. In particular, we seek to understand how herpes simplex virus, BK polyomavirus and Oropouche virus interact with and modify host cells to reprogram them into efficient factories for producing and disseminating new virions.
Our laboratory collaborates widely and employs a range of techniques to gain a multi-faceted understanding of virus replication, including advanced microscopy imaging, proteomics, and genome-wide genetic screens.
Publications
Key publications:
- Barker J, daSilva LLP, Crump CM. Mechanisms of bunyavirus morphogenesis and egress. J Gen Virol. 2023 Apr;104(4). doi: 10.1099/jgv.0.001845.
- Barbosa NS, Concha JO, daSilva LLP, Crump CM, Graham SC. Oropouche Virus Glycoprotein Topology and Cellular Requirements for Glycoprotein Secretion. J Virol. 2023 Jan 31;97(1):e0133122. doi: 10.1128/jvi.01331-22. Epub 2022 Dec 8.
- Nahas KL, Connor V, Scherer KM, Kaminski CF, Harkiolaki M, Crump CM, Graham SC. Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of multiple cellular organelles during HSV-1 infection. PLoS Pathog. 2022 Jul 7;18(7):e1010629. doi: 10.1371/journal.ppat.1010629. eCollection 2022 Jul.
- Scherer KM, Manton JD, Soh TK, Mascheroni L, Connor V, Crump CM, Kaminski CF. A fluorescent reporter system enables spatiotemporal analysis of host cell modification during herpes simplex virus-1 replication. J Biol Chem. 2021 Jan-Jun;296:100236. doi: 10.1074/jbc.RA120.016571. Epub 2021 Jan 7.
- Soh TK, Davies CTR, Muenzner J, Hunter LM, Barrow HG, Connor V, Bouton CR, Smith C, Emmott E, Antrobus R, Graham SC, Weekes MP, Crump CM. Whole-cell and plasma membrane proteomics of herpes simplex virus-1 infection reveals cell surface remodelling via pUL56-mediated GOPC degradation. Cell Rep. 2020 Oct 6;33(1):108235. doi: 10.1016/j.celrep.2020.108235.
- Caller LG, Davies CTR, Antrobus R, Lehner PJ, Weekes MP, Crump CM. Temporal Proteomic Analysis of BK Polyomavirus Infection Reveals Virus-Induced G2 Arrest and Highly Effective Evasion of Innate Immune Sensing. J J Virol. 2019 Jul 30;93(16):e00595-19. doi: 10.1128/JVI.00595-19. Print 2019 Aug 15.
- Albecka A, Owen DJ, Ivanova L, Brun J, Liman R, Davies L, Ahmed MF, Colaco S, Hollinshead M, Graham SC, Crump CM. Dual Function of the pUL7-pUL51 Tegument Protein Complex in Herpes Simplex Virus 1 Infection. J Virol. 2017 Jan 3;91(2):e02196-16. doi: 10.1128/JVI.02196-16. Print 2017 Jan 15.
- Albecka A, Laine RF, Janssen AFJ, Kaminski CF, Crump CM. HSV-1 glycoproteins are delivered to virus assembly sites through dynamin-dependent endocytosis. J Virol. 2017 Jan 3;91(2):e02196-16. doi: 10.1128/JVI.02196-16. Print 2017 Jan 15.
- Laine RF, Albecka A, van de Linde S, Rees EJ, Crump CM, Kaminski CF. Structural analysis of herpes simplex virus by optical super-resolution imaging. Nat Commun. 2015 Jan 22;6:5980. doi: 10.1038/ncomms6980.
