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PI Research

Ajioka Research

 
Brierley Research

Cryo-EM image of ribosomal-stalling at a frameshift-promoting RNA pseudoknot (purple). Shown are 40S subunit (yellow), bent tRNA (green) and associated eEF2 (red; Namy 2006 Nature 441 p244ff).

Coleman Research

The W12 cell line system is an accurate model of progression from low-grade pre-cancer (A) to squamous cell carcinoma (B).

Cooke Research

 
Crump Research 1

Figure 1: Electron micrograph of a herpes simplex virus type-1 (HSV-1) particle.

Crump Research 2

Figure 2: Immunofluorescence image of a cell infected with herpes simplex virus type-1 (HSV-1). The localisation of two different major viral structural proteins is shown in green and red

DAvino Research

A ring of Citron kinase (green) surrounding the microtubules (red) of a midbody isolated from human HeLa cells.

Doorbar Research

Our past work has identified important changes in viral gene expression that underlie the development of HPV-associated cervical intraepithelial neoplasias (top left). A series of molecular studies that we have published in the Journal of Virology over the last few years has revealed how one of the viral proteins (E4) is post-transcriptionally modified to facilitate its deposition as amyloid-like fibrils (bottom left). An image of E4 staining in a patient biopsy of a cervical neoplasia prepared using a cross-reactive E4 antibody prepared in the lab is shown in the MRC Strategic Plan www.mrc.ac.uk/strategicplan to illustrate to potential of such research in the development of diagnostic strategies for disease stratification.

Du Research

 
Dunne Research

 
Edwards Research

 
Ferguson Research

 
Firth Research 2

Fig 2. Influenza A virus genome map and synonymous site conservation analysis. Conservation peaks correspond to terminal packaging signals, splicing enhancer sequences, and the PA/X and NS1/NEP dual-coding region

Firth Research 1

Fig 1. Above - map of the poliovirus genome showing the polyprotein-encoding sequence (blue), UTRs (black), and known functional RNA elements (Goodfellow et al 2000, PMID 10775595; Liu et al 2009, PMID 19781674; Song et al 2012, PMID 22886087; Burrill et al 2013, PMID 23966409). Below - conservation at synonymous sites in an alignment of 50 enterovirus sequences: synonymous substitution rate relative to the genome average (brown) and statistical significance (red). All known functional RNA elements embedded within the polyprotein-encoding sequence are easily detected, besides a few additional elements in the region encoding 3D.

Fraser Research

Flagella on the surface of the bacterial cell (inset top right, Salmonella cell membrane stained green and flagella stained red, visualized by epifluorescence microscopy) comprise three contiguous substructures - the basal body, hook and filament - that are assembled sequentially. A central channel runs through these substructures to the flagellum tip. The basal body houses the flagellar export machinery (A) and the rod (FliE, FlgB, FlgC, FlgF and FlgG), which extends from the inner membrane (IM) and crosses the periplasm, peptidoglycan (PG) cell wall and outer membrane (OM). The cell surface hook (FlgE) is a flexible universal joint that connects the external flagellum filament (flagellin, FliC) to the basal body. (A) Subunits of the rod, hook and filament link head-to-tail at the cytoplasmic membrane export machinery. Subunits are first unfolded by the export ATPase complex (red) and then dock at the FlhB export gate (orange). The N-terminal helix of the docked subunit is then captured by the free C-terminal helix of an exiting subunit in the flagellum channel. (B) Sequential subunits are linked head-to-tail in a chain by juxtaposed terminal helices forming parallel coiled-coils. The resulting chain of unfolded subunits is connected through the flagellum central channel to the distal tip of the flagellum. (C) The subunits in the chain transit from the gate to the tip. Subunits fold and incorporate into the flagellum tip beneath cap foldases (FlgJ for rod, FlgD for hook and FliD for filament subunit assembly). Subunit folding and/or crystallization not only provides a strong anchor at the flagellum tip, it also shortens the chain in the channel thus exerting a pulling force on the next subunit at the export gate, pulling it from the gate. The pulling force then drops rapidly as the new unfolded subunit enters the channel. This process repeats for each subunit captured into the chain.

Graham Research

 
Holmes Research

 
Hughes Research 2

 
Hughes Research 1

 
Humphreys Research

 
Kaufman Research

 
Kelly Research

 
Koronakis Research 4

 
Koronakis Research 1

 
Koronakis Research 5

 
Kouzarides Research

 
Laman Research

Ribbon diagrams of the FP domain of PI31 in two dimerization configurations: through the alpha helical domain (upper pair), and through the beta sheet interface (lower pair). Kirk, et al. J. Biol. Chem. 2008. 283(32):22325-35

Moffett Research

 
Rawlins Research

Wholemount early stage embryonic lungs stained for Id2 mRNA (purple), which is located at the distal tips of the budding epithelium. The lungs form by progressive branching of an epithelial tube, which is surrounded by loosely packed mesenchymal cells.

Rudd Research

 
Smith Research

Image shows vaccinia virus particles (green) being pushed away from a cell on actin protrusions (red) from a cell that is infected but has not yet made new virus particles

Sweeney

 
Trowsdale Research

Receptors in the Leukocyte Receptor Complex and some proposed ligands

Turner Research

 
Watson Research

A mammary gland terminal end bud fluorescently stained with antibodies to the cell adhesion protein E-cadherin (green) and the marker of proliferation PCNA (red) with the nuclei of the cells in blue.

Xuereb Research

 
Wilson Research

 
Fraser Research 2

Flagella on the surface of the bacterial cell (inset top right, Salmonella cell membrane stained green and flagella stained red, visualized by epifluorescence microscopy) comprise three contiguous substructures - the basal body, hook and filament - that are assembled sequentially. A central channel runs through these substructures to the flagellum tip. The basal body houses the flagellar export machinery (A) and the rod (FliE, FlgB, FlgC, FlgF and FlgG), which extends from the inner membrane (IM) and crosses the periplasm, peptidoglycan (PG) cell wall and outer membrane (OM). The cell surface hook (FlgE) is a flexible universal joint that connects the external flagellum filament (flagellin, FliC) to the basal body. (A) Subunits of the rod, hook and filament link head-to-tail at the cytoplasmic membrane export machinery. Subunits are first unfolded by the export ATPase complex (red) and then dock at the FlhB export gate (orange). The N-terminal helix of the docked subunit is then captured by the free C-terminal helix of an exiting subunit in the flagellum channel. (B) Sequential subunits are linked head-to-tail in a chain by juxtaposed terminal helices forming parallel coiled-coils. The resulting chain of unfolded subunits is connected through the flagellum central channel to the distal tip of the flagellum. (C) The subunits in the chain transit from the gate to the tip. Subunits fold and incorporate into the flagellum tip beneath cap foldases (FlgJ for rod, FlgD for hook and FliD for filament subunit assembly). Subunit folding and/or crystallization not only provides a strong anchor at the flagellum tip, it also shortens the chain in the channel thus exerting a pulling force on the next subunit at the export gate, pulling it from the gate. The pulling force then drops rapidly as the new unfolded subunit enters the channel. This process repeats for each subunit captured into the chain.

Graham Research 2

 
Graham Research 2

 
affara-mouse-sperm

Figure 1 Mouse sperm nuclei stained with DAPI. The pink signal is from in situ hybridization of a Y specific DNA probe to the Y chromosome.

affara-epigentic-dna

Figure 3 The common epigenetic DNA modification represented by methylation of cytosine.

affara-foetus

 
affara-porcine

Figure 2 Good porcine mothering behaviour in the immediate postnatal period. Passive presentation of teats.

Du Research 2