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Dr Stephen Graham

Dr Stephen Graham

Sir Henry Dale Fellow and University Lecturer

Molecular mechanisms of membrane trafficking and viral infection

Department of Pathology
University of Cambridge
Tennis Court Road

Office Phone: +44 (0)1223 336920

Research themes


Molecular mechanisms of membrane trafficking and viral infection

Research Interests

Graham Research 2The compartmentalisation characteristic of eukaryotic cells presents a logistical dilemma: How do cells ensure components are efficiently delivered to only the correct intracellular compartment? My lab seeks to understand how multiprotein complexes regulate fusion of compartments in the eukaryotic endocytic pathway.

Viruses that seek to exploit the rich environment inside host cells face a similar dilemma: How can they subvert host membrane trafficking to ensure progeny virions efficiently exit infected cells? I am investigating how viruses that acquire double membrane envelopes within cells are trafficked to and fuse with the plasma membrane.

Lastly, in order to establish a fruitful infection viruses must evade the immune surveillance systems of the host. I am interested in how large double-stranded DNA viruses have hijacked proteins of the mammalian innate immune system and ‘reprogrammed’ these proteins to suit the needs of the virus.

  • Group Members: 
    Julia Muenzner, Danielle Owen, Susanna Colaco, Morag Hunter, Chen Gao, Lyudmila Ivanova

Key Publications

  1. L. Wartosch, U. Günesdogan, S.C. Graham, J.P. Luzio (2015) Recruitment of VPS33A to HOPS by VPS16 is required for lysosome fusion with endosomes and autophagosomes. Traffic16: 727–742
    doi: 10.1111/tra.12283
  2. S. Neidel, C. Maluquer de Motes, D.S. Mansur, P. Strnadova, G.L. Smith, S.C. Graham (2015) Vaccinia Virus Protein A49 is an Unexpected Member of the B-cell Lymphoma (Bcl)-2 Protein Family. Journal of Biological Chemistry, 290: 5991–6002
    doi: 10.1074/jbc.M114.624650
  3. B.T. Kelly, S.C. Graham, N. Liska, P.N. Dannhauser, S. Höning, E.J. Ungewickell, D.J. Owen (2014) AP2 controls clathrin polymerization with a membrane-activated switch. Science, 345: 459–463
    doi: 10.1126/science.1254836
  4. Y. Hackmann, S.C. Graham^, S. Ehl, S. Höning, K. Lehmberg, M. Aricò, D.J. Owen, G.M. Griffiths^ (2013) Syntaxin binding mechanism and disease-causing mutations in Munc18-2. Proceedings of the National Academy of Sciences of the USA, 110: E4482–E4491
    doi: 10.1073/pnas.1313474110
  5. S.C. Graham*^, L. Wartosch*, S.R. Gray, E.J. Scourfield, J.E. Deane, J.P. Luzio^, D.J. Owens (2013) Structural basis of Vps33A recruitment to the human HOPS complex by Vps16. Proceedings of the National Academy of Sciences of the USA, 110: 13345–13350
    doi: 10.1073/pnas.1307074110

(*Joint first authors, ^Joint corresponding authors)