Mike Clark
"An antibody engineer who also enjoys the mountains."
mrc7@cam.ac.uk
Mike's home-page
© Mike Clark, PhD., Cambridge University, UK
Last updated on 14th March 2000
Date: Thu, 9 Mar 2000 15:37:03 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-5144071: 121 aa
>cp1gh
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 58.3236+/-0.0111; mu= -169.1206+/- 0.481;
mean_var=11438.6422+/-9793.049, 0's: 1 Z-trim: 0 B-trim: 0 in 0/21
Kolmogorov-Smirnov statistic: 0.2425 (N=25) at 56
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.300
The best scores are: initn init1 opt z-sc E(637)
DP-34_DA-10 ( 302) [1] 522 522 522 83.5 4.9
DP-29_12-2 ( 302) [1] 511 511 511 82.5 5.6
VHD26 ( 302) [1] 483 483 483 79.8 7.7
DP-30 ( 302) [1] 479 479 479 79.5 8.1
V3-49 ( 302) [1] 469 469 476 79.2 8.4
HC15-7 ( 302) [1] 475 475 475 79.1 8.5
DP-57_HV3003- ( 302) [1] 465 465 473 78.9 8.7
DP-35_V3-11- ( 296) [1] 431 243 471 78.8 8.8
VH3-8 ( 294) [1] 441 253 469 78.7 9
LSG12-1 ( 302) [1] 463 463 470 78.6 9
DP-58_HV3D1EG ( 296) [1] 442 254 467 78.4 9.2
YAC-9_COS-27- ( 302) [1] 461 461 468 78.4 9.3
LSG6-1 ( 302) [1] 467 467 467 78.3 9.4
V3-22P ( 302) [1] 455 455 463 78.0 9.8
>>DP-34_DA-10 (302 aa)
Frame: 1 initn: 522 init1: 522 opt: 522 Z-score: 83.5 expect() 4.9
73.000% identity in 100 aa overlap (1-100:1-300)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.:.::::::::::::.:::::.:::::.:.::.:.:: ::. ::.::::.::.: ::
DP-34_ EVQLVESGGGLVQPGGSLRLSCAASGFTFSDYYMSWVRQAPGKGLEWVGFIRNKANGGTT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
: . :::::::::::..... :::::.:::::::.:::::
DP-34_ EXTTSVKGRFTISRDDSKSITYLQMNSLRAEDTAVYYCAR
190 220 250 280
cp1gh S
>>DP-29_12-2 (302 aa)
Frame: 1 initn: 511 init1: 511 opt: 511 Z-score: 82.5 expect() 5.6
72.000% identity in 100 aa overlap (1-100:1-300)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.:.::::::::::::.:::::.:::::.: ::.:.:: ::. ::.: :.::..:::
DP-29_ EVQLVESGGGLVQPGGSLRLSCAASGFTFSDHYMDWVRQAPGKGLEWVGRTRNKANSYTT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
:: :::::::::::...: ::::::.:..::::.:::::
DP-29_ EYAASVKGRFTISRDDSKNSLYLQMNSLKTEDTAVYYCAR
190 220 250 280
cp1gh S
>>VHD26 (302 aa)
Frame: 1 initn: 483 init1: 483 opt: 483 Z-score: 79.8 expect() 7.7
69.000% identity in 100 aa overlap (1-100:1-300)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.::::::::::::::.:::::.:::::.: ::.:.:: ::. : .:.::.::..:::
VHD26 EVQLLESGGGLVQPGGSLRLSCAASGFTFSDHYMSWVRQAQGKGLELVGLIRNKANSYTT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
:: :::::.::::....: :::::..:..:: :.:::::
VHD26 EYAASVKGRLTISREDSKNTLYLQMSSLKTEDLAVYYCAR
190 220 250 280
cp1gh S
>>DP-30 (302 aa)
Frame: 1 initn: 479 init1: 479 opt: 479 Z-score: 79.5 expect() 8.1
68.000% identity in 100 aa overlap (1-100:1-300)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.:.::::::::::::.:::::.:::::.: ::.:.:: ::. : .:.::.::..:::
DP-30 EVQLVESGGGLVQPGGSLRLSCAASGFTFSDHYMSWVRQAQGKGLELVGLIRNKANSYTT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
:: :::::.::::....: :::::..:..:: :.:::::
DP-30 EYAASVKGRLTISREDSKNTLYLQMSSLKTEDLAVYYCAR
190 220 250 280
cp1gh S
>>V3-49 (302 aa)
Frame: 1 initn: 469 init1: 469 opt: 476 Z-score: 79.2 expect() 8.4
68.000% identity in 100 aa overlap (1-100:1-300)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.:.:::::::::: :.::::..::::: :. :.:.:: ::. ::.::::.:: : ::
V3-49 EVQLVESGGGLVQPGRSLRLSCTASGFTFGDYAMSWFRQAPGKGLEWVGFIRSKAYGGTT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
::. :::::::::::..... :::::.:..::::.:::.:
V3-49 EYTASVKGRFTISRDGSKSIAYLQMNSLKTEDTAVYYCTR
190 220 250 280
cp1gh S
>>HC15-7 (302 aa)
Frame: 1 initn: 475 init1: 475 opt: 475 Z-score: 79.1 expect() 8.5
67.000% identity in 100 aa overlap (1-100:1-300)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.:.::::::::::::.:::::.:::::.: ::.:.:: ::. : .:.::.::..:::
HC15-7 EVQLVESGGGLVQPGGSLRLSCAASGFTFSDHYMSWVRQAQGKGLELVGLIRNKANSYTT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
:: :::::.::::....: .::::..:..:: :.:::::
HC15-7 EYAASVKGRLTISREDSKNTMYLQMSNLKTEDLAVYYCAR
190 220 250 280
cp1gh S
>>DP-57_HV3003- (302 aa)
Frame: 1 initn: 465 init1: 465 opt: 473 Z-score: 78.9 expect() 8.7
67.000% identity in 100 aa overlap (1-100:1-300)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.:.::::::::::::.:::::.:::::. .::. .:: ::. ::.::::.::.: ::
DP-57_ EVQLVESGGGLVQPGGSLRLSCAASGFTFSYYYMSGVRQAPGKGLEWVGFIRNKANGGTT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
: . :::::::::::..... ::::..:..::::.:::.:
DP-57_ EXTTSVKGRFTISRDDSKSITYLQMKSLKTEDTAVYYCSR
190 220 250 280
cp1gh S
>>DP-35_V3-11- (296 aa)
Frame: 1 initn: 431 init1: 243 opt: 471 Z-score: 78.8 expect() 8.8
69.000% identity in 100 aa overlap (1-100:1-294)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
.:.:.:::::::.::::.:::::.:::::.:.::.:::: ::. ::...: ...: :
DP-35_ QVQLVESGGGLVKPGGSLRLSCAASGFTFSDYYMSWIRQAPGKGLEWVSYI--SSSGSTI
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
: ::::::::::::..: ::::::.:::::::.:::::
DP-35_ YYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCAR
190 220 250 280
cp1gh S
>>VH3-8 (294 aa)
Frame: 1 initn: 441 init1: 253 opt: 469 Z-score: 78.7 expect() 9
69.000% identity in 100 aa overlap (1-100:1-294)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
.:.:::::::::.::::.:::::.:::::.:.::.:::: ::. ::...: .... :
VH3-8 QVQLLESGGGLVKPGGSLRLSCAASGFTFSDYYMSWIRQAPGKGLEWVSYISSSSS--YT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
.: ::::::::::::..: ::::::.:::::::.:::::
VH3-8 NYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCAR
190 220 250 280
cp1gh S
>>LSG12-1 (302 aa)
Frame: 1 initn: 463 init1: 463 opt: 470 Z-score: 78.6 expect() 9
67.000% identity in 100 aa overlap (1-100:1-300)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.:.:::::::::: :.::::..::::: . :.:.:: ::. ::.::::.:: : ::
LSG12- EVQLVESGGGLVQPGPSLRLSCTASGFTFGYYPMSWVRQAPGKGLEWVGFIRSKAYGGTT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
:: :::::::::::..... :::::.:..::::.:::.:
LSG12- EYAASVKGRFTISRDDSKSIAYLQMNSLKTEDTAVYYCTR
190 220 250 280
cp1gh S
>>DP-58_HV3D1EG (296 aa)
Frame: 1 initn: 442 init1: 254 opt: 467 Z-score: 78.4 expect() 9.2
69.000% identity in 100 aa overlap (1-100:1-294)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.:.::::::::::::.:::::.:::::... :::.:: ::. ::...: ...: :
DP-58_ EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYEMNWVRQAPGKGLEWVSYI--SSSGSTI
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
: ::::::::::::..: ::::::.:::::::.:::::
DP-58_ YYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCAR
190 220 250 280
cp1gh S
>>YAC-9_COS-27- (302 aa)
Frame: 1 initn: 461 init1: 461 opt: 468 Z-score: 78.4 expect() 9.3
66.000% identity in 100 aa overlap (1-100:1-300)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.:.::::::::::::..::::.:::::. :.:.:: .::. ::.: ::.::..:.:
YAC-9_ EVQLVESGGGLVQPGGSLKLSCAASGFTFSGSAMHWVRQASGKGLEWVGRIRSKANSYAT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
: :::::::::::...: :::::.:..::::.:::.:
YAC-9_ AYAASVKGRFTISRDDSKNTAYLQMNSLKTEDTAVYYCTR
190 220 250 280
cp1gh S
>>LSG6-1 (302 aa)
Frame: 1 initn: 467 init1: 467 opt: 467 Z-score: 78.3 expect() 9.4
66.327% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.:.:::::::.::::.:::::.:::::.. .:::.:: ::. ::.: :..:. : ::
LSG6-1 EVQLVESGGGLVKPGGSLRLSCAASGFTFSNAWMNWVRQAPGKGLEWVGRIKSKTDGGTT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
.: ::::::::::...: ::::::.:..::::.:::.
LSG6-1 DYAAPVKGRFTISRDDSKNTLYLQMNSLKTEDTAVYYCTT
190 220 250 280
cp1gh S
>>V3-22P (302 aa)
Frame: 1 initn: 455 init1: 455 opt: 463 Z-score: 78.0 expect() 9.8
66.000% identity in 100 aa overlap (1-100:1-300)
10 20 30 40 50 60
cp1gh EVKLLESGGGLVQPGGSMRLSCAGSGFTFTDFYMNWIRQPAGKAPEWLGFIRDKAKGYTT
::.:.::::.:::::::.:::::.:::::. .::. .:: ::. ::.::::.::.: ::
V3-22P EVHLVESGGALVQPGGSLRLSCAASGFTFSYYYMSGVRQAPGKGLEWVGFIRNKANGGTT
10 40 70 100 130 160
70 80 90 100 110 120
cp1gh EYNPSVKGRFTISRDNTQNMLYLQMNTLRAEDTATYYCAREGHTAAPFDYWGQGVMVTVS
: . :::::::::::..... ::::..:..::::.:::.:
V3-22P EXTTSVKGRFTISRDDSKSITYLQMKSLKTEDTAVYYCSR
190 220 250 280
cp1gh S
121 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Thu Mar 9 15:37:02 2000 done: Thu Mar 9 15:37:03 2000
Scan time: 0.300 Display time: 0.030
Function used was TFASTA
Date: Thu, 9 Mar 2000 18:09:31 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-5508063: 21 aa
>cp1ghj
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 3.9599+/-0.00831; mu= 1.2877+/- 0.468;
mean_var=10.5398+/- 7.724, 0's: 2 Z-trim: 11 B-trim: 0 in 0/17
Kolmogorov-Smirnov statistic: 0.2173 (N=26) at 44
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.150
The best scores are: initn init1 opt z-sc E(625)
JH4D ( 48) [3] 90 90 90 276.0 9e-11
JH4B ( 48) [3] 90 90 90 276.0 9e-11
JH4A ( 48) [3] 90 90 90 276.0 9e-11
JH3B ( 50) [2] 85 85 85 260.1 6.9e-10
JH3A ( 50) [2] 85 85 85 260.1 6.9e-10
JH5A ( 51) [3] 80 80 80 244.5 5.2e-09
JH5B ( 51) [3] 79 79 79 241.4 7.6e-09
JH1 ( 52) [1] 66 66 77 235.0 1.7e-08
JH2 ( 53) [2] 75 75 75 228.6 3.9e-08
JH6A ( 63) [3] 62 62 69 208.0 5.5e-07
JH6B ( 63) [3] 62 62 69 208.0 5.5e-07
JH6C ( 63) [3] 57 57 64 192.6 4e-06
>>JH4D (48 aa)
Frame: 3 initn: 90 init1: 90 opt: 90 Z-score: 276.0 expect() 9e-11
85.714% identity in 14 aa overlap (8-21:6-47)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
:::::::..:::::
JH4D YFDYWGQGTLVTVSS
30
>>JH4B (48 aa)
Frame: 3 initn: 90 init1: 90 opt: 90 Z-score: 276.0 expect() 9e-11
85.714% identity in 14 aa overlap (8-21:6-47)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
:::::::..:::::
JH4B YFDYWGQGTLVTVSS
30
>>JH4A (48 aa)
Frame: 3 initn: 90 init1: 90 opt: 90 Z-score: 276.0 expect() 9e-11
85.714% identity in 14 aa overlap (8-21:6-47)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
:::::::..:::::
JH4A YFDYWGQGTLVTVSS
30
>>JH3B (50 aa)
Frame: 2 initn: 85 init1: 85 opt: 85 Z-score: 260.1 expect() 6.9e-10
85.714% identity in 14 aa overlap (8-21:8-49)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
:: ::::.::::::
JH3B DAFDIWGQGTMVTVSS
20
>>JH3A (50 aa)
Frame: 2 initn: 85 init1: 85 opt: 85 Z-score: 260.1 expect() 6.9e-10
85.714% identity in 14 aa overlap (8-21:8-49)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
:: ::::.::::::
JH3A DAFDVWGQGTMVTVSS
20
>>JH5A (51 aa)
Frame: 3 initn: 80 init1: 80 opt: 80 Z-score: 244.5 expect() 5.2e-09
78.571% identity in 14 aa overlap (8-21:9-50)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
:: ::::..:::::
JH5A NWFDSWGQGTLVTVSS
30
>>JH5B (51 aa)
Frame: 3 initn: 79 init1: 79 opt: 79 Z-score: 241.4 expect() 7.6e-09
78.571% identity in 14 aa overlap (8-21:9-50)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
:: ::::..:::::
JH5B NWFDPWGQGTLVTVSS
30
>>JH1 (52 aa)
Frame: 1 initn: 66 init1: 66 opt: 77 Z-score: 235.0 expect() 1.7e-08
64.706% identity in 17 aa overlap (5-21:1-51)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
: :..::::..:::::
JH1 AEYFQHWGQGTLVTVSS
10 40
>>JH2 (53 aa)
Frame: 2 initn: 75 init1: 75 opt: 75 Z-score: 228.6 expect() 3.9e-08
71.429% identity in 14 aa overlap (8-21:11-52)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
:: ::.:..:::::
JH2 YWYFDLWGRGTLVTVSS
20 50
>>JH6A (63 aa)
Frame: 3 initn: 62 init1: 62 opt: 69 Z-score: 208.0 expect() 5.5e-07
76.923% identity in 13 aa overlap (9-21:24-62)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
: ::::. :::::
JH6A YYYYYGMDVWGQGTTVTVSS
30 60
>>JH6B (63 aa)
Frame: 3 initn: 62 init1: 62 opt: 69 Z-score: 208.0 expect() 5.5e-07
76.923% identity in 13 aa overlap (9-21:24-62)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
: ::::. :::::
JH6B YYYYYGMDVWGQGTTVTVSS
30 60
>>JH6C (63 aa)
Frame: 3 initn: 57 init1: 57 opt: 64 Z-score: 192.6 expect() 4e-06
69.231% identity in 13 aa overlap (9-21:24-62)
10 20
cp1ghj EGHTAAPFDYWGQGVMVTVSS
: ::.:. :::::
JH6C YYYYYYMDVWGKGTTVTVSS
30 60
21 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Thu Mar 9 18:09:30 2000 done: Thu Mar 9 18:09:31 2000
Scan time: 0.150 Display time: 0.020
Function used was TFASTA
Date: Thu, 9 Mar 2000 15:38:26 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-5216932: 108 aa
>mrc7@cam.ac.uk
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 40.6350+/- 0.013; mu= -124.6895+/- 0.618;
mean_var=10515.5456+/-9197.943, 0's: 1 Z-trim: 0 B-trim: 0 in 0/21
Kolmogorov-Smirnov statistic: 0.2044 (N=25) at 42
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.260
The best scores are: initn init1 opt z-sc E(637)
DPK1_O18- ( 287) [1] 466 466 494 87.9 2.8
DPK9_O12- ( 287) [1] 458 458 492 87.7 2.8
V3B ( 287) [1] 469 469 492 87.7 2.8
DPK4_A20 ( 287) [1] 451 451 480 86.5 3.3
DPK8_VD ( 287) [1] 449 449 479 86.4 3.3
A30_SG3 ( 287) [1] 462 462 473 85.9 3.6
V108 ( 287) [1] 463 463 472 85.8 3.6
HK137 ( 287) [1] 437 437 472 85.8 3.6
LFVK431 ( 287) [1] 433 433 468 85.4 3.8
VB ( 287) [1] 439 439 468 85.4 3.8
DPK5_VB ( 287) [1] 439 439 468 85.4 3.8
DPK6_VB ( 287) [1] 439 439 468 85.4 3.8
DPK3_L11 ( 287) [1] 451 451 466 85.2 3.9
DPK2_L14 ( 287) [1] 444 444 464 85.0 4
Z4 ( 287) [1] 432 432 463 84.9 4.1
DPK7_HK134- ( 287) [1] 424 424 459 84.5 4.3
HK102_V1 ( 287) [1] 433 433 459 84.5 4.3
DPK11_O14- ( 287) [1] 453 453 459 84.5 4.3
VA ( 287) [1] 415 415 457 84.3 4.4
L12A_PCRDIL6-5 ( 287) [1] 431 431 457 84.3 4.4
>>DPK1_O18- (287 aa)
Frame: 1 initn: 466 init1: 466 opt: 494 Z-score: 87.9 expect() 2.8
74.737% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: ::::::::::..:.:::.:..:::::::: :..::::::...::.::.::
DPK1_O DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYDASNLETGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
::::::::::::.:::::::::.:::.: :. . :
DPK1_O RFSGSGSGTDFTFTISSLQPEDIATYYCQQYDNLP
190 220 250 280
>>DPK9_O12- (287 aa)
Frame: 1 initn: 458 init1: 458 opt: 492 Z-score: 87.7 expect() 2.8
75.789% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: ::::::::::..:.:::.:..:::::::: :..:::::: ...::.:.::
DPK9_O DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::::::::::::::: :::.: : : :
DPK9_O RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTP
190 220 250 280
>>V3B (287 aa)
Frame: 1 initn: 469 init1: 469 opt: 492 Z-score: 87.7 expect() 2.8
75.789% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::::::::::::::..:.:::.:..:::::::: :..:::::: ...::.:.::
V3B DIQMTQSPSFLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::::::::::::::: :::.: : :
V3B RFSGSGSGTDFTLTISSLQPEDFATYYCQCGYSTP
190 220 250 280
>>DPK4_A20 (287 aa)
Frame: 1 initn: 451 init1: 451 opt: 480 Z-score: 86.5 expect() 3.3
74.737% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: ::::::::::..:.:::.:..:: ::::: :. :::::: ...::.:.::
DPK4_A DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWYQQKPGKVPKLLIYAASTLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
::::::::::::::::::::::::::.: .. : :
DPK4_A RFSGSGSGTDFTLTISSLQPEDVATYYCQKYNSAP
190 220 250 280
>>DPK8_VD (287 aa)
Frame: 1 initn: 449 init1: 449 opt: 479 Z-score: 86.4 expect() 3.3
73.684% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::..::::::::::::::::..:.:::.:..:: ::::: :..:::::: ...::.:.::
DPK8_V DIQLTQSPSFLSASVGDRVTITCRASQGISSYLAWYQQKPGKAPKLLIYAASTLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::.:::::::::::: :::.: : : :
DPK8_V RFSGSGSGTEFTLTISSLQPEDFATYYCQQLNSYP
190 220 250 280
>>A30_SG3 (287 aa)
Frame: 1 initn: 462 init1: 462 opt: 473 Z-score: 85.9 expect() 3.6
73.684% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: ::::::::::..:.:::.: . :.::::: :..:: ::: ...::.:.::
A30_SG DIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKRLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::.:::::::::::: :::.:::: : :
A30_SG RFSGSGSGTEFTLTISSLQPEDFATYYCLQHNSYP
190 220 250 280
>>V108 (287 aa)
Frame: 1 initn: 463 init1: 463 opt: 472 Z-score: 85.8 expect() 3.6
72.632% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::..::::: ::::::::::..:.:::.:.. :.::::: :..: :::: ...::.:.::
V108 DIQVTQSPSSLSASVGDRVTITCRASQGISNGLSWYQQKPGQAPTLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
::::::::::::::::::::::::::.::: . :
V108 RFSGSGSGTDFTLTISSLQPEDVATYYCLQDYTTP
190 220 250 280
>>HK137 (287 aa)
Frame: 1 initn: 437 init1: 437 opt: 472 Z-score: 85.8 expect() 3.6
72.632% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: ::::::::::..:.:::.:..:: :.::: :..:: ::: ...::.:.::
HK137 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWFQQKPGKAPKSLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::::::::::::::: :::.: :. : :
HK137 RFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
190 220 250 280
>>LFVK431 (287 aa)
Frame: 1 initn: 433 init1: 433 opt: 468 Z-score: 85.4 expect() 3.8
71.579% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: ::::::::::..:.:::.:..:: :.::: :..:: ::: ...::.:.::
LFVK43 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWFQQKPGKAPKSLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
.::::::::::::::::::::: :::.: :. : :
LFVK43 KFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
190 220 250 280
>>VB (287 aa)
Frame: 1 initn: 439 init1: 439 opt: 468 Z-score: 85.4 expect() 3.8
72.632% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: .:::::::::..:.:::.:...: ::::: :..:::::: ...::.:.::
VB DIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::::::::::::::: :::.: : : :
VB RFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFP
190 220 250 280
>>DPK5_VB (287 aa)
Frame: 1 initn: 439 init1: 439 opt: 468 Z-score: 85.4 expect() 3.8
72.632% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: .:::::::::..:.:::.:...: ::::: :..:::::: ...::.:.::
DPK5_V DIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::::::::::::::: :::.: : : :
DPK5_V RFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFP
190 220 250 280
>>DPK6_VB (287 aa)
Frame: 1 initn: 439 init1: 439 opt: 468 Z-score: 85.4 expect() 3.8
72.632% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: .:::::::::..:.:::.:...: ::::: :..:::::: ...::.:.::
DPK6_V DIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::::::::::::::: :::.: : : :
DPK6_V RFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFP
190 220 250 280
>>DPK3_L11 (287 aa)
Frame: 1 initn: 451 init1: 451 opt: 466 Z-score: 85.2 expect() 3.9
73.404% identity in 94 aa overlap (2-95:4-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
:.:::::: ::::::::::..:.:::.: . :.::::: :..:::::: ...::.:.::
DPK3_L AIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::::::::::::::: :::.::: . :
DPK3_L RFSGSGSGTDFTLTISSLQPEDFATYYCLQDYNYP
190 220 250 280
>>DPK2_L14 (287 aa)
Frame: 1 initn: 444 init1: 444 opt: 464 Z-score: 85.0 expect() 4
70.526% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
.:.:::::: .:::::::::..:.: :.:..:: :.::: :. :: ::: ...::.:.::
DPK2_L NIQMTQSPSAMSASVGDRVTITCRARQGISNYLAWFQQKPGKVPKHLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::.:::::::::::: :::.:::: : :
DPK2_L RFSGSGSGTEFTLTISSLQPEDFATYYCLQHNSYP
190 220 250 280
>>Z4 (287 aa)
Frame: 1 initn: 432 init1: 432 opt: 463 Z-score: 84.9 expect() 4.1
72.632% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: ::::::::::..:.:::.:.. ::::::: :..::.::: ...::.::::
Z4 DIQMTQSPSSLSASVGDRVTITCRASQGISNNLNWYQQKPGKTPKFLIYAASSLQSGIPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
::: ::::::.::::::::::: :::.: : : :
Z4 RFSDSGSGTDYTLTISSLQPEDFATYYCQQSDSTP
190 220 250 280
>>DPK7_HK134- (287 aa)
Frame: 1 initn: 424 init1: 424 opt: 459 Z-score: 84.5 expect() 4.3
71.579% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: ::::::::::..:.:::.:...: ::::: ..:: ::: ...::.:.::
DPK7_H DIQMTQSPSSLSASVGDRVTITCRASQGISSWLAWYQQKPEKAPKSLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::::::::::::::: :::.: :. : :
DPK7_H RFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
190 220 250 280
>>HK102_V1 (287 aa)
Frame: 1 initn: 433 init1: 433 opt: 459 Z-score: 84.5 expect() 4.3
70.968% identity in 93 aa overlap (1-93:1-279)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: ::::::::::..:.:::.:...: ::::: :..::::::....:..:.::
HK102_ DIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYDASSLESGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::.::::::::::.: :::.: :. :
HK102_ RFSGSGSGTEFTLTISSLQPDDFATYYCQQYNSYS
190 220 250 280
>>DPK11_O14- (287 aa)
Frame: 1 initn: 453 init1: 453 opt: 459 Z-score: 84.5 expect() 4.3
71.579% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::..::::: ::::::::::..:..::.:..:::::.:: :. ::::::...:::.:.::
DPK11_ DIQLTQSPSSLSASVGDRVTITCRVSQGISSYLNWYRQKPGKVPKLLIYSASNLQSGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
::::::::::::::::::::::::::. . . :
DPK11_ RFSGSGSGTDFTLTISSLQPEDVATYYGQRTYNAP
190 220 250 280
>>VA (287 aa)
Frame: 1 initn: 415 init1: 415 opt: 457 Z-score: 84.3 expect() 4.4
71.277% identity in 94 aa overlap (2-95:4-285)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
:..::::: ::::::::::..:.:::.:.. : ::::: :..::::::....:..:.::
VA AIQLTQSPSSLSASVGDRVTITCRASQGISSALAWYQQKPGKAPKLLIYDASSLESGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::::::::::::::: :::.: : : :
VA RFSGSGSGTDFTLTISSLQPEDFATYYCQQFNSYP
190 220 250 280
>>L12A_PCRDIL6-5 (287 aa)
Frame: 1 initn: 431 init1: 431 opt: 457 Z-score: 84.3 expect() 4.4
70.968% identity in 93 aa overlap (1-93:1-279)
10 20 30 40 50 60
cp1gl DIKMTQSPSFLSASVGDRVTLNCKASQNIDKYLNWYQQKLGESPKLLIYNTNNLQTGIPS
::.:::::: ::::::::::..:.:::.:...: ::::: :..::::::....:..:.::
L12A_P DIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYKASSLESGVPS
10 40 70 100 130 160
70 80 90 100
cp1gl RFSGSGSGTDFTLTISSLQPEDVATYFCLQHISRPRTFGTGTKLELKR
:::::::::.::::::::::.: :::.: :. :
L12A_P RFSGSGSGTEFTLTISSLQPDDFATYYCQQYNSYS
190 220 250 280
108 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Thu Mar 9 15:38:26 2000 done: Thu Mar 9 15:38:26 2000
Scan time: 0.260 Display time: 0.030
Function used was TFASTA
Date: Thu, 9 Mar 2000 18:11:04 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-5147508: 13 aa
>cp1glj
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 1.4627+/-0.00917; mu= 9.2358+/- 0.518;
mean_var=11.2187+/- 8.682, 0's: 18 Z-trim: 6 B-trim: 8 in 1/18
Kolmogorov-Smirnov statistic: 0.1797 (N=21) at 58
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.130
The best scores are: initn init1 opt z-sc E(614)
JK2 ( 39) [3] 50 50 58 179.6 2.1e-05
JK5 ( 38) [2] 47 47 55 170.7 6.5e-05
JK1 ( 38) [2] 39 39 54 167.8 9.5e-05
JK4 ( 38) [2] 38 38 53 164.8 0.00014
JK3 ( 38) [2] 39 39 50 155.8 0.00044
>>JK2 (39 aa)
Frame: 3 initn: 50 init1: 50 opt: 58 Z-score: 179.6 expect() 2.1e-05
81.818% identity in 11 aa overlap (2-12:6-38)
10
cp1glj RTFGTGTKLELKR
::: :::::.:
JK2 YTFGQGTKLEIK
30
>>JK5 (38 aa)
Frame: 2 initn: 47 init1: 47 opt: 55 Z-score: 170.7 expect() 6.5e-05
72.727% identity in 11 aa overlap (2-12:5-37)
10
cp1glj RTFGTGTKLELKR
::: ::.::.:
JK5 ITFGQGTRLEIK
20
>>JK1 (38 aa)
Frame: 2 initn: 39 init1: 39 opt: 54 Z-score: 167.8 expect() 9.5e-05
72.727% identity in 11 aa overlap (2-12:5-37)
10
cp1glj RTFGTGTKLELKR
::: :::.:.:
JK1 WTFGQGTKVEIK
20
>>JK4 (38 aa)
Frame: 2 initn: 38 init1: 38 opt: 53 Z-score: 164.8 expect() 0.00014
72.727% identity in 11 aa overlap (2-12:5-37)
10
cp1glj RTFGTGTKLELKR
::: :::.:.:
JK4 LTFGGGTKVEIK
20
>>JK3 (38 aa)
Frame: 2 initn: 39 init1: 39 opt: 50 Z-score: 155.8 expect() 0.00044
63.636% identity in 11 aa overlap (2-12:5-37)
10
cp1glj RTFGTGTKLELKR
::: :::...:
JK3 FTFGPGTKVDIK
20
Mike Clark