Mike Clark
"An antibody engineer who also enjoys the mountains."
mrc7@cam.ac.uk
Mike's home-page
© Mike Clark, PhD., Cambridge University, UK
Last updated on 11th July 2001
LOCUS MUSIGHMX 406 bp mRNA ROD 21-OCT-1993
DEFINITION Mouse Ig rearranged gamma-chain (G-2a) mRNA V-region (V-J2),
partial cds.
ACCESSION M28251
VERSION M28251.1 GI:195663
KEYWORDS constant region; immunoglobulin gamma-chain; immunoglobulin heavy
chain; joining region; processed gene; variable region.
SOURCE Mouse (strain BALB/c) Anti-Tac hybridoma, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia;
Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.
REFERENCE 1 (bases 1 to 406)
AUTHORS Queen,C.
JOURNAL Unpublished (1989)
REFERENCE 2 (sites)
AUTHORS Queen,C., Schneider,W.P., Selick,H.E., Payne,P.W., Landolfi,N.F.,
Duncan,J.F., Avdalovic,N.M., Levitt,M., Junghans,R.P. and
Waldmann,T.A.
TITLE A humanized antibody that binds to the interleukin 2 receptor
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 86 (24), 10029-10033 (1989)
MEDLINE 90099290
COMMENT [2] sites; for [1].
Draft entry and computer-readable sequence for [1] kindly submitted
by C.Queen, 22-SEP-1989.
FEATURES Location/Qualifiers
source 1..406
/organism="Mus musculus"
/db_xref="taxon:10090"
sig_peptide 1..58
/note="Ig gamma-chain signal peptide"
CDS 1..>406
/note="Ig gamma 2a-chain V-region; (V-J2) precursor"
/codon_start=1
/protein_id="AAA03013.1"
/db_xref="GI:195664"
/translation="MERHWIFLFLFSVTAGVHSQVQLQQSGAELAKPGASVKMSCKAS
GYTFTSYRMHWVKQRPGQGLEWIGYINPSTGYTEYNQKFKDKATLTADKSSSTAYMQL
SSLTFEDSAVYYCARGGGVFDYWGQGTTLTVSSX"
mat_peptide 59..>406
/note="Ig gamma chain"
misc_recomb 351..352
/note="V-region end/N-region start"
misc_recomb 362..363
/note="N-region end/J2-region start"
BASE COUNT 105 a 100 c 106 g 95 t
ORIGIN
1 atggaaaggc actggatctt tctcttcctg ttttcagtaa ctgcaggtgt ccactcccag
61 gtccagcttc agcagtctgg ggctgaactg gcaaaacctg gggcctcagt gaagatgtcc
121 tgcaaggctt ctggctacac ctttactagc tacaggatgc actgggtaaa acagaggcct
181 ggacagggtc tggaatggat tggatatatt aatcctagca ctgggtatac tgaatacaat
241 cagaagttca aggacaaggc cacattgact gcagacaaat cctccagcac agcctacatg
301 caactgagca gcctgacatt tgaggactct gcagtctatt actgtgcaag aggggggggg
361 gtctttgact actggggcca aggaaccact ctcacagtct cctcag
//
LOCUS MUSIGLBA 385 bp mRNA ROD 21-OCT-1993
DEFINITION Mouse rearranged light chain mRNA V-region (V-J5), partial cds.
ACCESSION M28250
VERSION M28250.1 GI:197695
KEYWORDS constant region; immunoglobulin light chain; joining region;
processed gene; variable region.
SOURCE Mouse (strain BALB/c) Anti-Tac hybridoma, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia;
Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.
REFERENCE 1 (bases 1 to 385)
AUTHORS Queen,C.
JOURNAL Unpublished (1989)
REFERENCE 2 (sites)
AUTHORS Queen,C., Schneider,W.P., Selick,H.E., Payne,P.W., Landolfi,N.F.,
Duncan,J.F., Avdalovic,N.M., Levitt,M., Junghans,R.P. and
Waldmann,T.A.
TITLE A humanized antibody that binds to the interleukin 2 receptor
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 86 (24), 10029-10033 (1989)
MEDLINE 90099290
COMMENT [2] sites; for [1].
Draft entry and computer-readable sequence for [1] kindly submitted
by C.Queen, 22-SEP-1989.
FEATURES Location/Qualifiers
source 1..385
/organism="Mus musculus"
/db_xref="taxon:10090"
CDS 1..>385
/note="Ig light chain V-region; (V-J5) precursor"
/codon_start=1
/protein_id="AAA03012.1"
/db_xref="GI:197696"
/translation="MHFQVQIFSFLLISASVIMSRGQIVLTQSPAIMSASPGEKVTIT
CSASSSISYMHWFQQKPGTSPKLWIYTTSNLASGVPARFSGSGSGTSYSLTISRMEAE
DAATYYCHQRSTYPLTFGSGTKLELKX"
sig_peptide 1..66
/note="Ig light chain signal peptide"
mat_peptide 67..>385
/note="Ig light chain"
misc_recomb 348..349
/note="V-region end/J5-region start"
BASE COUNT 96 a 109 c 82 g 98 t
ORIGIN
1 atgcattttc aagtgcagat tttcagcttc ctgctaatca gtgcctcagt cataatgtcc
61 agaggacaaa ttgttctcac ccagtctcca gcaatcatgt ctgcatctcc aggggagaag
121 gtcaccataa cctgcagtgc cagctcaagt ataagttaca tgcactggtt ccagcagaag
181 ccaggcactt ctcccaaact ctggatttat accacatcca acctggcttc tggagtccct
241 gctcgcttca gtggcagtgg atctgggacc tcttactctc tcacaatcag ccgaatggag
301 gctgaagatg ctgccactta ttactgccat caaaggagta cttacccact cacgttcggt
361 tctgggacca agctggagct gaaac
//
Date: Wed, 15 Mar 2000 00:25:57 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-5907723: 116 aa
>aTacH
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 64.4609+/- 0.011; mu= -191.3890+/- 0.490;
mean_var=9996.0198+/-8217.330, 0's: 1 Z-trim: 0 B-trim: 0 in 0/21
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.300
The best scores are: initn init1 opt z-sc E(637)
HV1F10T ( 296) [1] 493 493 493 81.8 6.1
DP-7_21-2- ( 296) [1] 493 493 493 81.8 6.1
HG3 ( 296) [1] 488 488 488 81.3 6.5
DP-75_VI-2- ( 296) [1] 481 481 481 80.6 7.1
VHGL1-2 ( 296) [1] 480 480 480 80.5 7.1
DP-25_VI-3B ( 296) [1] 477 477 477 80.2 7.4
DP-8 ( 296) [1] 475 475 475 80.0 7.6
V35_VI-2B ( 296) [1] 467 467 467 79.2 8.4
1-1 ( 296) [1] 466 466 466 79.1 8.5
HA2 ( 296) [1] 462 462 462 78.7 9
>>HV1F10T (296 aa)
Frame: 1 initn: 493 init1: 493 opt: 493 Z-score: 81.8 expect() 6.1
73.469% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
aTacH QVQLQQSGAELAKPGASVKMSCKASGYTFTSYRMHWVKQRPGQGLEWIGYINPSTGYTEY
:::: :::::. :::::::.:::::::::::: ::::.: :::::::.: :::: : : :
HV1F10 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGIINPSGGSTSY
10 40 70 100 130 160
70 80 90 100 110
aTacH NQKFKDKATLTADKSSSTAYMQLSSLTFEDSAVYYCARGGGVFDYWGQGTTLTVSS
:::. ..:.: : :.::.::.:::: ::.:::::::
HV1F10 AQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR
190 220 250 280
>>DP-7_21-2- (296 aa)
Frame: 1 initn: 493 init1: 493 opt: 493 Z-score: 81.8 expect() 6.1
73.469% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
aTacH QVQLQQSGAELAKPGASVKMSCKASGYTFTSYRMHWVKQRPGQGLEWIGYINPSTGYTEY
:::: :::::. :::::::.:::::::::::: ::::.: :::::::.: :::: : : :
DP-7_2 QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGIINPSGGSTSY
10 40 70 100 130 160
70 80 90 100 110
aTacH NQKFKDKATLTADKSSSTAYMQLSSLTFEDSAVYYCARGGGVFDYWGQGTTLTVSS
:::. ..:.: : :.::.::.:::: ::.:::::::
DP-7_2 AQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR
190 220 250 280
>>HG3 (296 aa)
Frame: 1 initn: 488 init1: 488 opt: 488 Z-score: 81.3 expect() 6.5
72.449% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
aTacH QVQLQQSGAELAKPGASVKMSCKASGYTFTSYRMHWVKQRPGQGLEWIGYINPSTGYTEY
:::: :::::. :::::::.:::::::::.:: ::::.: :::::::.: :::: : : :
HG3 QVQLVQSGAEVKKPGASVKVSCKASGYTFNSYYMHWVRQAPGQGLEWMGIINPSGGSTSY
10 40 70 100 130 160
70 80 90 100 110
aTacH NQKFKDKATLTADKSSSTAYMQLSSLTFEDSAVYYCARGGGVFDYWGQGTTLTVSS
:::. ..:.: : :.::.::.:::: ::.:::::::
HG3 AQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCAR
190 220 250 280
>>DP-75_VI-2- (296 aa)
Frame: 1 initn: 481 init1: 481 opt: 481 Z-score: 80.6 expect() 7.1
70.408% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
aTacH QVQLQQSGAELAKPGASVKMSCKASGYTFTSYRMHWVKQRPGQGLEWIGYINPSTGYTEY
:::: :::::. :::::::.::::::::::.: ::::.: :::::::.:.:::..: :.:
DP-75_ QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGWINPNSGGTNY
10 40 70 100 130 160
70 80 90 100 110
aTacH NQKFKDKATLTADKSSSTAYMQLSSLTFEDSAVYYCARGGGVFDYWGQGTTLTVSS
:::. ..:.: : : :::::.:: : .:.:::::::
DP-75_ AQKFQGRVTMTRDTSISTAYMELSRLRSDDTAVYYCAR
190 220 250 280
>>VHGL1-2 (296 aa)
Frame: 1 initn: 480 init1: 480 opt: 480 Z-score: 80.5 expect() 7.1
70.408% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
aTacH QVQLQQSGAELAKPGASVKMSCKASGYTFTSYRMHWVKQRPGQGLEWIGYINPSTGYTEY
:::: :::::. :::::::.:::::::::::: ::::.: :::::::.:.:::..: :.:
VHGL1- QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGWINPNSGGTNY
10 40 70 100 130 160
70 80 90 100 110
aTacH NQKFKDKATLTADKSSSTAYMQLSSLTFEDSAVYYCARGGGVFDYWGQGTTLTVSS
:::. ..:.: : : ::::..:: : .:.:::::::
VHGL1- AQKFQGRVTMTRDTSISTAYVELSRLRSDDTAVYYCAR
190 220 250 280
>>DP-25_VI-3B (296 aa)
Frame: 1 initn: 477 init1: 477 opt: 477 Z-score: 80.2 expect() 7.4
71.429% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
aTacH QVQLQQSGAELAKPGASVKMSCKASGYTFTSYRMHWVKQRPGQGLEWIGYINPSTGYTEY
:::: :::::. :::::::.:::::::::::: ::::.: ::: :::.:.:: ..: :.:
DP-25_ QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYAMHWVRQAPGQRLEWMGWINAGNGNTKY
10 40 70 100 130 160
70 80 90 100 110
aTacH NQKFKDKATLTADKSSSTAYMQLSSLTFEDSAVYYCARGGGVFDYWGQGTTLTVSS
.:::. ..:.: : :.:::::.:::: ::.:::::::
DP-25_ SQKFQGRVTITRDTSASTAYMELSSLRSEDTAVYYCAR
190 220 250 280
>>DP-8 (296 aa)
Frame: 1 initn: 475 init1: 475 opt: 475 Z-score: 80.0 expect() 7.6
70.408% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
aTacH QVQLQQSGAELAKPGASVKMSCKASGYTFTSYRMHWVKQRPGQGLEWIGYINPSTGYTEY
:::: :::::. :::::::.::::::::::.: ::::.: :::::::.:.:::..: :.:
DP-8 QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGWINPNSGGTNY
10 40 70 100 130 160
70 80 90 100 110
aTacH NQKFKDKATLTADKSSSTAYMQLSSLTFEDSAVYYCARGGGVFDYWGQGTTLTVSS
:::. .:.: : : :::::.:: : .:.:::::::
DP-8 AQKFQGWVTMTRDTSISTAYMELSRLRSDDTAVYYCAR
190 220 250 280
>>V35_VI-2B (296 aa)
Frame: 1 initn: 467 init1: 467 opt: 467 Z-score: 79.2 expect() 8.4
69.388% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
aTacH QVQLQQSGAELAKPGASVKMSCKASGYTFTSYRMHWVKQRPGQGLEWIGYINPSTGYTEY
:::: :::::. :::::::.::::::::::.: ::::.: :::::::.: :::..: :.:
V35_VI QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMHWVRQAPGQGLEWMGRINPNSGGTNY
10 40 70 100 130 160
70 80 90 100 110
aTacH NQKFKDKATLTADKSSSTAYMQLSSLTFEDSAVYYCARGGGVFDYWGQGTTLTVSS
:::. ..: : : : :::::.:: : .:..::::::
V35_VI AQKFQGRVTSTRDTSISTAYMELSRLRSDDTVVYYCAR
190 220 250 280
>>1-1 (296 aa)
Frame: 1 initn: 466 init1: 466 opt: 466 Z-score: 79.1 expect() 8.5
69.388% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
aTacH QVQLQQSGAELAKPGASVKMSCKASGYTFTSYRMHWVKQRPGQGLEWIGYINPSTGYTEY
:::: :::::. : :::::.::::::::::.: ::::.: :::::::.:.:::..: :.:
1-1 QVQLVQSGAEVKKLGASVKVSCKASGYTFTGYYMHWVQQAPGQGLEWMGWINPNSGGTNY
10 40 70 100 130 160
70 80 90 100 110
aTacH NQKFKDKATLTADKSSSTAYMQLSSLTFEDSAVYYCARGGGVFDYWGQGTTLTVSS
:::. ..:.: : : :::::.:: : .:.:::::::
1-1 AQKFQGRVTMTRDTSISTAYMELSRLRSDDTAVYYCAR
190 220 250 280
>>HA2 (296 aa)
Frame: 1 initn: 462 init1: 462 opt: 462 Z-score: 78.7 expect() 9
68.367% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
aTacH QVQLQQSGAELAKPGASVKMSCKASGYTFTSYRMHWVKQRPGQGLEWIGYINPSTGYTEY
:::: ::::. :::::::.:::::::::::: :.:..: ::::.::.:.:: ..: :.:
HA2 QVQLVXSGAEVKKPGASVKVSCKASGYTFTSYYMNWMRQAPGQGFEWMGWINAGNGNTKY
10 40 70 100 130 160
70 80 90 100 110
aTacH NQKFKDKATLTADKSSSTAYMQLSSLTFEDSAVYYCARGGGVFDYWGQGTTLTVSS
.::.. ..:.: : :.:::::::::: ::.:::::::
HA2 SQKLQGRVTITRDTSASTAYMQLSSLRSEDTAVYYCAR
190 220 250 280
116 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Wed Mar 15 00:25:57 2000 done: Wed Mar 15 00:25:57 2000
Scan time: 0.300 Display time: 0.020
Function used was TFASTA
Date: Wed, 15 Mar 2000 00:27:40 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-5768952: 18 aa
>aTacHJ
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 3.6040+/-0.00704; mu= 4.9437+/- 0.394;
mean_var=14.0501+/-10.263, 0's: 2 Z-trim: 12 B-trim: 0 in 0/18
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.160
The best scores are: initn init1 opt z-sc E(624)
JH4D ( 48) [3] 87 87 87 231.7 2.7e-08
JH4B ( 48) [3] 87 87 87 231.7 2.7e-08
JH4A ( 48) [3] 87 87 87 231.7 2.7e-08
JH3B ( 50) [2] 78 78 78 207.3 6.1e-07
JH3A ( 50) [2] 78 78 78 207.3 6.1e-07
JH5A ( 51) [3] 77 77 77 204.4 8.8e-07
JH5B ( 51) [3] 76 76 76 201.8 1.2e-06
JH6B ( 63) [3] 69 69 76 199.7 1.6e-06
JH6A ( 63) [3] 69 69 76 199.7 1.6e-06
JH1 ( 52) [1] 63 63 73 193.6 3.5e-06
>>JH4D (48 aa)
Frame: 3 initn: 87 init1: 87 opt: 87 Z-score: 231.7 expect() 2.7e-08
85.714% identity in 14 aa overlap (5-18:6-47)
10
aTacHJ GGGVFDYWGQGTTLTVSS
:::::::: .::::
JH4D YFDYWGQGTLVTVSS
30
>>JH4B (48 aa)
Frame: 3 initn: 87 init1: 87 opt: 87 Z-score: 231.7 expect() 2.7e-08
85.714% identity in 14 aa overlap (5-18:6-47)
10
aTacHJ GGGVFDYWGQGTTLTVSS
:::::::: .::::
JH4B YFDYWGQGTLVTVSS
30
>>JH4A (48 aa)
Frame: 3 initn: 87 init1: 87 opt: 87 Z-score: 231.7 expect() 2.7e-08
85.714% identity in 14 aa overlap (5-18:6-47)
10
aTacHJ GGGVFDYWGQGTTLTVSS
:::::::: .::::
JH4A YFDYWGQGTLVTVSS
30
>>JH3B (50 aa)
Frame: 2 initn: 78 init1: 78 opt: 78 Z-score: 207.3 expect() 6.1e-07
78.571% identity in 14 aa overlap (5-18:8-49)
10
aTacHJ GGGVFDYWGQGTTLTVSS
:: ::::: .::::
JH3B DAFDIWGQGTMVTVSS
20
>>JH3A (50 aa)
Frame: 2 initn: 78 init1: 78 opt: 78 Z-score: 207.3 expect() 6.1e-07
78.571% identity in 14 aa overlap (5-18:8-49)
10
aTacHJ GGGVFDYWGQGTTLTVSS
:: ::::: .::::
JH3A DAFDVWGQGTMVTVSS
20
>>JH5A (51 aa)
Frame: 3 initn: 77 init1: 77 opt: 77 Z-score: 204.4 expect() 8.8e-07
78.571% identity in 14 aa overlap (5-18:9-50)
10
aTacHJ GGGVFDYWGQGTTLTVSS
:: ::::: .::::
JH5A NWFDSWGQGTLVTVSS
30
>>JH5B (51 aa)
Frame: 3 initn: 76 init1: 76 opt: 76 Z-score: 201.8 expect() 1.2e-06
78.571% identity in 14 aa overlap (5-18:9-50)
10
aTacHJ GGGVFDYWGQGTTLTVSS
:: ::::: .::::
JH5B NWFDPWGQGTLVTVSS
30
>>JH6B (63 aa)
Frame: 3 initn: 69 init1: 69 opt: 76 Z-score: 199.7 expect() 1.6e-06
84.615% identity in 13 aa overlap (6-18:24-62)
10
aTacHJ GGGVFDYWGQGTTLTVSS
: ::::::.::::
JH6B YYYYYGMDVWGQGTTVTVSS
30 60
>>JH6A (63 aa)
Frame: 3 initn: 69 init1: 69 opt: 76 Z-score: 199.7 expect() 1.6e-06
84.615% identity in 13 aa overlap (6-18:24-62)
10
aTacHJ GGGVFDYWGQGTTLTVSS
: ::::::.::::
JH6A YYYYYGMDVWGQGTTVTVSS
30 60
>>JH1 (52 aa)
Frame: 1 initn: 63 init1: 63 opt: 73 Z-score: 193.6 expect() 3.5e-06
71.429% identity in 14 aa overlap (5-18:10-51)
10
aTacHJ GGGVFDYWGQGTTLTVSS
:..::::: .::::
JH1 AEYFQHWGQGTLVTVSS
10 40
18 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Wed Mar 15 00:27:40 2000 done: Wed Mar 15 00:27:40 2000
Scan time: 0.160 Display time: 0.010
Function used was TFASTA
Date: Wed, 15 Mar 2000 00:31:17 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-5507190: 106 aa
>aTacL
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 52.3592+/-0.0104; mu= -148.9491+/- 0.488;
mean_var=7726.0206+/-6432.403, 0's: 1 Z-trim: 0 B-trim: 0 in 0/21
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.310
The best scores are: initn init1 opt z-sc E(637)
VG_38K- ( 287) [1] 396 299 421 81.1 6.6
DPK26_A26- ( 287) [1] 380 279 405 79.3 8.3
3A9 ( 290) [1] 294 294 404 79.1 8.5
VG_13K11- ( 287) [1] 390 293 400 78.7 8.9
DPK20_A11- ( 290) [1] 274 274 400 78.7 9
DPK8_VD ( 287) [1] 304 304 398 78.5 9.2
DPK25_A14 ( 287) [1] 328 231 396 78.3 9.4
HK137 ( 287) [1] 377 290 393 77.9 9.9
DPK22_A27- ( 290) [1] 273 273 393 77.9 9.9
DPK9_O12- ( 287) [1] 283 283 392 77.8 10
>>VG_38K- (287 aa)
Frame: 1 initn: 396 init1: 299 opt: 421 Z-score: 81.1 expect() 6.6
63.158% identity in 95 aa overlap (1-94:1-285)
10 20 30 40 50
aTacL QIVLTQSPAIMSASPGEKVTITCSASSSI-SYMHWFQQKPGTSPKLWIYTTSNLASGVPA
.:::::::: .: ::::..:..: ::.:. ::. :.::::: .:.: :: .:: :.:.::
VG_38K EIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPA
10 40 70 100 130 160
60 70 80 90 100
aTacL RFSGSGSGTSYSLTISRMEAEDAATYYCHQRSTYPLTFGSGTKLELK
:::::::::...:::: .: :: :.:::.:::..:
VG_38K RFSGSGSGTDFTLTISSLEPEDFAVYYCQQRSNWP
190 220 250 280
>>DPK26_A26- (287 aa)
Frame: 1 initn: 380 init1: 279 opt: 405 Z-score: 79.3 expect() 8.3
66.316% identity in 95 aa overlap (1-94:1-285)
10 20 30 40 50
aTacL QIVLTQSPAIMSASPGEKVTITCSASSSI-SYMHWFQQKPGTSPKLWIYTTSNLASGVPA
.::::::: ..:..: ::::::: ::.:: : .::.:::: :::: : .:. ::::.
DPK26_ EIVLTQSPDFQSVTPKEKVTITCRASQSIGSSLHWYQQKPDQSPKLLIKYASQSFSGVPS
10 40 70 100 130 160
60 70 80 90 100
aTacL RFSGSGSGTSYSLTISRMEAEDAATYYCHQRSTYPLTFGSGTKLELK
:::::::::...:::. .:::::::::::: :. :
DPK26_ RFSGSGSGTDFTLTINSLEAEDAATYYCHQSSSLP
190 220 250 280
>>3A9 (290 aa)
Frame: 1 initn: 294 init1: 294 opt: 404 Z-score: 79.1 expect() 8.5
61.053% identity in 95 aa overlap (1-93:1-285)
10 20 30 40 50
aTacL QIVLTQSPAIMSASPGEKVTITCSASSSI--SYMHWFQQKPGTSPKLWIYTTSNLASGVP
.:::::::: .: ::::..:..: ::.:. ::. :.::::: .:.: :: .:. :.:.:
3A9 EIVLTQSPATLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYDASSRATGIP
10 40 70 100 130 160
60 70 80 90 100
aTacL ARFSGSGSGTSYSLTISRMEAEDAATYYCHQRSTYPLTFGSGTKLELK
:::::::::...:::::.: :: :.:::.:::..
3A9 DRFSGSGSGTDFTLTISRLEPEDFAVYYCQQRSNWH
190 220 250 280
>>VG_13K11- (287 aa)
Frame: 1 initn: 390 init1: 293 opt: 400 Z-score: 78.7 expect() 8.9
60.638% identity in 94 aa overlap (1-93:1-282)
10 20 30 40 50
aTacL QIVLTQSPAIMSASPGEKVTITCSASSSI-SYMHWFQQKPGTSPKLWIYTTSNLASGVPA
.:::::::: .: ::::..:..: ::... ::. :.::::: .:.: :: .:: :.:.::
VG_13K EIVLTQSPATLSLSPGERATLSCRASQGVSSYLAWYQQKPGQAPRLLIYDASNRATGIPA
10 40 70 100 130 160
60 70 80 90 100
aTacL RFSGSGSGTSYSLTISRMEAEDAATYYCHQRSTYPLTFGSGTKLELK
:::::: ::...:::: .: :: :.:::.:::..
VG_13K RFSGSGPGTDFTLTISSLEPEDFAVYYCQQRSNWH
190 220 250 280
>>DPK20_A11- (290 aa)
Frame: 1 initn: 274 init1: 274 opt: 400 Z-score: 78.7 expect() 9
59.375% identity in 96 aa overlap (1-94:1-288)
10 20 30 40 50
aTacL QIVLTQSPAIMSASPGEKVTITCSASSSI--SYMHWFQQKPGTSPKLWIYTTSNLASGVP
.:::::::: .: ::::..:..:.::.:. ::. :.::::: .:.: :: .:. :.:.:
DPK20_ EIVLTQSPATLSLSPGERATLSCGASQSVSSSYLAWYQQKPGLAPRLLIYDASSRATGIP
10 40 70 100 130 160
60 70 80 90 100
aTacL ARFSGSGSGTSYSLTISRMEAEDAATYYCHQRSTYPLTFGSGTKLELK
:::::::::...:::::.: :: :.:::.: .. :
DPK20_ DRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSP
190 220 250 280
>>DPK8_VD (287 aa)
Frame: 1 initn: 304 init1: 304 opt: 398 Z-score: 78.5 expect() 9.2
63.830% identity in 94 aa overlap (2-94:4-285)
10 20 30 40 50
aTacL QIVLTQSPAIMSASPGEKVTITCSASSSI-SYMHWFQQKPGTSPKLWIYTTSNLASGVPA
: :::::...::: :..::::: ::..: ::. :.::::: .::: ::..:.: ::::.
DPK8_V DIQLTQSPSFLSASVGDRVTITCRASQGISSYLAWYQQKPGKAPKLLIYAASTLQSGVPS
10 40 70 100 130 160
60 70 80 90 100
aTacL RFSGSGSGTSYSLTISRMEAEDAATYYCHQRSTYPLTFGSGTKLELK
::::::::: ..:::: .. :: :::::.: ..::
DPK8_V RFSGSGSGTEFTLTISSLQPEDFATYYCQQLNSYP
190 220 250 280
>>DPK25_A14 (287 aa)
Frame: 1 initn: 328 init1: 231 opt: 396 Z-score: 78.3 expect() 9.4
61.702% identity in 94 aa overlap (2-94:4-285)
10 20 30 40 50
aTacL QIVLTQSPAIMSASPGEKVTITCSASSSI-SYMHWFQQKPGTSPKLWIYTTSNLASGVPA
.:.:::::..:..:::::::::.:: .: .:..:.:::: .::: : .:. ::::.
DPK25_ DVVMTQSPAFLSVTPGEKVTITCQASEGIGNYLYWYQQKPDQAPKLLIKYASQSISGVPS
10 40 70 100 130 160
60 70 80 90 100
aTacL RFSGSGSGTSYSLTISRMEAEDAATYYCHQRSTYPLTFGSGTKLELK
:::::::::....::: .::::::::::.: . .:
DPK25_ RFSGSGSGTDFTFTISSLEAEDAATYYCQQGNKHP
190 220 250 280
>>HK137 (287 aa)
Frame: 1 initn: 377 init1: 290 opt: 393 Z-score: 77.9 expect() 9.9
62.766% identity in 94 aa overlap (2-94:4-285)
10 20 30 40 50
aTacL QIVLTQSPAIMSASPGEKVTITCSASSSIS-YMHWFQQKPGTSPKLWIYTTSNLASGVPA
: .::::. .::: :..::::: ::..:: :. ::::::: .:: ::..:.: ::::.
HK137 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWFQQKPGKAPKSLIYAASSLQSGVPS
10 40 70 100 130 160
60 70 80 90 100
aTacL RFSGSGSGTSYSLTISRMEAEDAATYYCHQRSTYPLTFGSGTKLELK
:::::::::...:::: .. :: :::::.: ..::
HK137 RFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
190 220 250 280
>>DPK22_A27- (290 aa)
Frame: 1 initn: 273 init1: 273 opt: 393 Z-score: 77.9 expect() 9.9
58.333% identity in 96 aa overlap (1-94:1-288)
10 20 30 40 50
aTacL QIVLTQSPAIMSASPGEKVTITCSASSSI--SYMHWFQQKPGTSPKLWIYTTSNLASGVP
.:::::::. .: ::::..:..: ::.:. ::. :.::::: .:.: :: .:. :.:.:
DPK22_ EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIP
10 40 70 100 130 160
60 70 80 90 100
aTacL ARFSGSGSGTSYSLTISRMEAEDAATYYCHQRSTYPLTFGSGTKLELK
:::::::::...:::::.: :: :.:::.: .. :
DPK22_ DRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSP
190 220 250 280
>>DPK9_O12- (287 aa)
Frame: 1 initn: 283 init1: 283 opt: 392 Z-score: 77.8 expect() 10
62.766% identity in 94 aa overlap (2-94:4-285)
10 20 30 40 50
aTacL QIVLTQSPAIMSASPGEKVTITCSASSSI-SYMHWFQQKPGTSPKLWIYTTSNLASGVPA
: .::::. .::: :..::::: ::.:: ::..:.::::: .::: ::..:.: ::::.
DPK9_O DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
60 70 80 90 100
aTacL RFSGSGSGTSYSLTISRMEAEDAATYYCHQRSTYPLTFGSGTKLELK
:::::::::...:::: .. :: :::::.: . :
DPK9_O RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTP
190 220 250 280
106 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Wed Mar 15 00:31:16 2000 done: Wed Mar 15 00:31:17 2000
Scan time: 0.310 Display time: 0.020
Function used was TFASTA
Date: Wed, 15 Mar 2000 00:34:46 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-5795707: 12 aa
>aTacLJ
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 3.2107+/-0.00903; mu= 1.1371+/- 0.509;
mean_var=24.7522+/-18.538, 0's: 13 Z-trim: 5 B-trim: 9 in 1/19
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.150
The best scores are: initn init1 opt z-sc E(620)
JK4 ( 38) [2] 45 45 60 144.8 0.0018
JK2 ( 39) [3] 51 51 59 142.7 0.0024
JK5 ( 38) [2] 48 48 58 140.8 0.003
JK1 ( 38) [2] 40 40 55 134.8 0.0066
JK3 ( 38) [2] 39 39 51 126.7 0.018
JL6B ( 38) [2] 40 40 41 106.6 0.24
JL2_JL3A ( 38) [2] 40 40 41 106.6 0.24
JL3B ( 38) [2] 40 40 40 104.6 0.32
JL1 ( 38) [2] 37 37 38 100.6 0.53
O5 ( 231) [1] 38 38 38 89.0 2.3
>>JK4 (38 aa)
Frame: 2 initn: 45 init1: 45 opt: 60 Z-score: 144.8 expect() 0.0018
75.000% identity in 12 aa overlap (1-12:2-37)
10
aTacLJ LTFGSGTKLELK
::::.:::.:.:
JK4 LTFGGGTKVEIK
20
>>JK2 (39 aa)
Frame: 3 initn: 51 init1: 51 opt: 59 Z-score: 142.7 expect() 0.0024
81.818% identity in 11 aa overlap (2-12:6-38)
10
aTacLJ LTFGSGTKLELK
:::.:::::.:
JK2 YTFGQGTKLEIK
30
>>JK5 (38 aa)
Frame: 2 initn: 48 init1: 48 opt: 58 Z-score: 140.8 expect() 0.003
66.667% identity in 12 aa overlap (1-12:2-37)
10
aTacLJ LTFGSGTKLELK
.:::.::.::.:
JK5 ITFGQGTRLEIK
20
>>JK1 (38 aa)
Frame: 2 initn: 40 init1: 40 opt: 55 Z-score: 134.8 expect() 0.0066
72.727% identity in 11 aa overlap (2-12:5-37)
10
aTacLJ LTFGSGTKLELK
:::.:::.:.:
JK1 WTFGQGTKVEIK
20
>>JK3 (38 aa)
Frame: 2 initn: 39 init1: 39 opt: 51 Z-score: 126.7 expect() 0.018
58.333% identity in 12 aa overlap (1-12:2-37)
10
aTacLJ LTFGSGTKLELK
.::: :::...:
JK3 FTFGPGTKVDIK
20
>>JL6B (38 aa)
Frame: 2 initn: 40 init1: 40 opt: 41 Z-score: 106.6 expect() 0.24
85.714% identity in 7 aa overlap (3-9:8-28)
10
aTacLJ LTFGSGTKLELK
::::::.
JL6B NVFGSGTKVTVL
20
>>JL2_JL3A (38 aa)
Frame: 2 initn: 40 init1: 40 opt: 41 Z-score: 106.6 expect() 0.24
66.667% identity in 9 aa overlap (1-9:2-28)
10
aTacLJ LTFGSGTKLELK
..::.::::
JL2_JL VVFGGGTKLTVL
20
>>JL3B (38 aa)
Frame: 2 initn: 40 init1: 40 opt: 40 Z-score: 104.6 expect() 0.32
85.714% identity in 7 aa overlap (3-9:8-28)
10
aTacLJ LTFGSGTKLELK
::.::::
JL3B WVFGGGTKLTVL
20
>>JL1 (38 aa)
Frame: 2 initn: 37 init1: 37 opt: 38 Z-score: 100.6 expect() 0.53
71.429% identity in 7 aa overlap (3-9:8-28)
10
aTacLJ LTFGSGTKLELK
::.:::.
JL1 YVFGTGTKVTVL
20
>>O5 (231 aa)
Frame: 1 initn: 38 init1: 38 opt: 38 Z-score: 89.0 expect() 2.3
66.667% identity in 9 aa overlap (4-12:166-192)
10
aTacLJ LTFGSGTKLELK
::::.. ::
O5 YLQKPGQSPQLLIYEVSNQASEFSDRFRGNGSGTEFTLKVSRTETKDVGVYX
100 130 160 190 220
12 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Wed Mar 15 00:34:46 2000 done: Wed Mar 15 00:34:46 2000
Scan time: 0.150 Display time: 0.010
Function used was TFASTA
Mike Clark