Mike Clark
"An antibody engineer who also enjoys the mountains."
mrc7@cam.ac.uk
Mike's home-page
© Mike Clark, PhD., Cambridge University, UK
Last updated on 21st July 2000
>gi|1435159|emb|CAA67618.1| immunoglobulin heavy chain variable domain [Mus musculus] QVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIWPGDGDTNYNGKFKGKATL TADESSSTAYMQLSSLASEDSAVYFCARRETTTVGRYYYAMDYWGQGTSVTVSS
>gi|1435161|emb|CAA67621.1| immunoglobulin light chain variable domain [Mus musculus] DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPDRFTGSGSATD FTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
Date: Fri, 21 Jul 2000 15:36:21 +0100
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-1136836: 124 aa
>HD37H
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 60.5443+/- 0.011; mu= -174.9734+/- 0.484;
mean_var=11130.1783+/-9243.552, 0's: 1 Z-trim: 0 B-trim: 0 in 0/21
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.310
The best scores are: initn init1 opt z-sc E(637)
6M27 ( 296) [1] 465 465 465 78.0 9.8
>>6M27 (296 aa)
Frame: 1 initn: 465 init1: 465 opt: 465 Z-score: 78.0 expect() 9.8
69.388% identity in 98 aa overlap (1-98:1-294)
10 20 30 40 50 60
HD37H QVQLQQSGAELVRPGSSVKISCKASGYAFSSYWMNWVKQRPGQGLEWIGQIWPGDGDTNY
:::: :::::. .::::::.:::::: .:::: ..::.: :::::::.:.: : : .::
6M27 QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGRIIPILGTANY
10 40 70 100 130 160
70 80 90 100 110 120
HD37H NGKFKGKATLTADESSSTAYMQLSSLASEDSAVYFCARRETTTVGRYYYAMDYWGQGTSV
::.:..:.:::::.:::::.:::: :::.:::.:::
6M27 AQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCAR
190 220 250 280
HD37H TVSS
124 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Fri Jul 21 15:36:21 2000 done: Fri Jul 21 15:36:21 2000
Scan time: 0.310 Display time: 0.010
Function used was TFASTA
Date: Fri, 21 Jul 2000 15:43:09 +0100
Subject: FASTA results: HD37HJ
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-1158756: 26 aa
>HD37HJ
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 3.0928+/-0.00779; mu= 6.5481+/- 0.438;
mean_var=13.7628+/- 9.343, 0's: 2 Z-trim: 11 B-trim: 2 in 1/18
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.160
The best scores are: initn init1 opt z-sc E(625)
JH6A ( 63) [3] 108 108 108 288.9 1.7e-11
JH6B ( 63) [3] 108 108 108 288.9 1.7e-11
JH6C ( 63) [3] 101 101 101 270.1 1.9e-10
JH4D ( 48) [3] 81 81 81 218.4 1.5e-07
JH4B ( 48) [3] 81 81 81 218.4 1.5e-07
JH4A ( 48) [3] 81 81 81 218.4 1.5e-07
JH3B ( 50) [2] 66 66 78 210.0 4.3e-07
JH3A ( 50) [2] 66 66 78 210.0 4.3e-07
JH2 ( 53) [2] 59 59 74 198.7 1.8e-06
JH5A ( 51) [3] 65 65 71 191.0 4.9e-06
JH5B ( 51) [3] 65 65 70 188.3 7e-06
JH1 ( 52) [1] 65 65 67 180.0 2e-05
>>JH6A (63 aa)
Frame: 3 initn: 108 init1: 108 opt: 108 Z-score: 288.9 expect() 1.7e-11
83.333% identity in 18 aa overlap (9-26:9-62)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
:::.:: :::::.:::::
JH6A YYYYYGMDVWGQGTTVTVSS
30 60
>>JH6B (63 aa)
Frame: 3 initn: 108 init1: 108 opt: 108 Z-score: 288.9 expect() 1.7e-11
83.333% identity in 18 aa overlap (9-26:9-62)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
:::.:: :::::.:::::
JH6B YYYYYGMDVWGQGTTVTVSS
30 60
>>JH6C (63 aa)
Frame: 3 initn: 101 init1: 101 opt: 101 Z-score: 270.1 expect() 1.9e-10
77.778% identity in 18 aa overlap (9-26:9-62)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
::: :: ::.::.:::::
JH6C YYYYYYMDVWGKGTTVTVSS
30 60
>>JH4D (48 aa)
Frame: 3 initn: 81 init1: 81 opt: 81 Z-score: 218.4 expect() 1.5e-07
92.308% identity in 13 aa overlap (14-26:9-47)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
::::::: :::::
JH4D YFDYWGQGTLVTVSS
30
>>JH4B (48 aa)
Frame: 3 initn: 81 init1: 81 opt: 81 Z-score: 218.4 expect() 1.5e-07
92.308% identity in 13 aa overlap (14-26:9-47)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
::::::: :::::
JH4B YFDYWGQGTLVTVSS
30
>>JH4A (48 aa)
Frame: 3 initn: 81 init1: 81 opt: 81 Z-score: 218.4 expect() 1.5e-07
92.308% identity in 13 aa overlap (14-26:9-47)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
::::::: :::::
JH4A YFDYWGQGTLVTVSS
30
>>JH3B (50 aa)
Frame: 2 initn: 66 init1: 66 opt: 78 Z-score: 210.0 expect() 4.3e-07
80.000% identity in 15 aa overlap (12-26:5-49)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
:.: ::::: :::::
JH3B DAFDIWGQGTMVTVSS
20
>>JH3A (50 aa)
Frame: 2 initn: 66 init1: 66 opt: 78 Z-score: 210.0 expect() 4.3e-07
80.000% identity in 15 aa overlap (12-26:5-49)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
:.: ::::: :::::
JH3A DAFDVWGQGTMVTVSS
20
>>JH2 (53 aa)
Frame: 2 initn: 59 init1: 59 opt: 74 Z-score: 198.7 expect() 1.8e-06
64.706% identity in 17 aa overlap (10-26:2-52)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
:. .: ::.:: :::::
JH2 YWYFDLWGRGTLVTVSS
20 50
>>JH5A (51 aa)
Frame: 3 initn: 65 init1: 65 opt: 71 Z-score: 191.0 expect() 4.9e-06
84.615% identity in 13 aa overlap (14-26:12-50)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
: ::::: :::::
JH5A NWFDSWGQGTLVTVSS
30
>>JH5B (51 aa)
Frame: 3 initn: 65 init1: 65 opt: 70 Z-score: 188.3 expect() 7e-06
84.615% identity in 13 aa overlap (14-26:12-50)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
: ::::: :::::
JH5B NWFDPWGQGTLVTVSS
30
>>JH1 (52 aa)
Frame: 1 initn: 65 init1: 65 opt: 67 Z-score: 180.0 expect() 2e-05
83.333% identity in 12 aa overlap (15-26:16-51)
10 20
HD37HJ RETTTVGRYYYAMDYWGQGTSVTVSS
.::::: :::::
JH1 AEYFQHWGQGTLVTVSS
10 40
26 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Fri Jul 21 15:43:09 2000 done: Fri Jul 21 15:43:09 2000
Scan time: 0.160 Display time: 0.020
Function used was TFASTA
Date: Fri, 21 Jul 2000 15:37:25 +0100
Subject: FASTA results: HD37L
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-1162793: 108 aa
>HD37L
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 39.2494+/-0.0122; mu= -115.2375+/- 0.562;
mean_var=11462.3488+/-10004.814, 0's: 1 Z-trim: 0 B-trim: 0 in 0/21
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.250
The best scores are: initn init1 opt z-sc E(637)
DPK9_O12- ( 287) [1] 424 424 432 80.4 7.2
L10A ( 290) [1] 349 262 425 79.7 7.9
DPK7_HK134- ( 287) [1] 423 423 423 79.5 8.1
V3B ( 287) [1] 414 414 422 79.4 8.2
V268 ( 290) [1] 344 262 420 79.2 8.4
DPK23_L25- ( 290) [1] 344 262 420 79.2 8.4
DPK6_VB ( 287) [1] 369 369 419 79.2 8.4
VB ( 287) [1] 369 369 419 79.2 8.4
DPK5_VB ( 287) [1] 369 369 419 79.2 8.4
DPK1_O18- ( 287) [1] 379 379 417 79.0 8.7
HK101_HK146- ( 287) [1] 417 417 417 79.0 8.7
V108 ( 287) [1] 368 368 416 78.9 8.8
VG_38K- ( 287) [1] 368 368 416 78.9 8.8
VE ( 287) [1] 412 412 416 78.9 8.8
HK137 ( 287) [1] 414 414 414 78.7 9
DPK8_VD ( 287) [1] 412 412 412 78.5 9.2
DPK3_L11 ( 287) [1] 406 406 411 78.4 9.3
DPK24_VKIVKLOB- ( 305) [1] 388 301 413 78.4 9.3
LFVK431 ( 287) [1] 410 410 410 78.3 9.4
VH ( 290) [1] 336 249 408 78.1 9.7
>>DPK9_O12- (287 aa)
Frame: 1 initn: 424 init1: 424 opt: 432 Z-score: 80.4 expect() 7.2
64.211% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::.:.: :::.:::.::.::... .:..:::::: ..::::::.::. .:::.
DPK9_O DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: :.:: :
DPK9_O RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTP
190 220 250 280
>>L10A (290 aa)
Frame: 1 initn: 349 init1: 262 opt: 425 Z-score: 79.7 expect() 7.9
64.583% identity in 96 aa overlap (1-95:1-288)
10 20 30 40 50
HD37L DILITQSPKSMSMSVGERVTLTCKASENV-VTYVSWYQQKPEQSPKLLIYGASNRYTGVP
.:..:::: ..:.: ::::::.:.::..: .:.::::::: :.:.:::::::.: ::.:
L10A EIVMTQSPPTLSLSPGERVTLSCRASQSVSSSYLSWYQQKPGQAPRLLIYGASTRATGIP
10 40 70 100 130 160
60 70 80 90 100
HD37L DRFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: : :. :
L10A ARFSGSGSGTDFTLTISSLQPEDFAVYYCQQDYNLP
190 220 250 280
>>DPK7_HK134- (287 aa)
Frame: 1 initn: 423 init1: 423 opt: 423 Z-score: 79.5 expect() 8.1
63.158% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::.:.: :::.:::.::.::... ....:::::::..:: :::.::. .:::.
DPK7_H DIQMTQSPSSLSASVGDRVTITCRASQGISSWLAWYQQKPEKAPKSLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: : :::
DPK7_H RFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
190 220 250 280
>>V3B (287 aa)
Frame: 1 initn: 414 init1: 414 opt: 422 Z-score: 79.4 expect() 8.2
63.158% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::. .: :::.:::.::.::... .:..:::::: ..::::::.::. .:::.
V3B DIQMTQSPSFLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: ::: :
V3B RFSGSGSGTDFTLTISSLQPEDFATYYCQCGYSTP
190 220 250 280
>>V268 (290 aa)
Frame: 1 initn: 344 init1: 262 opt: 420 Z-score: 79.2 expect() 8.4
63.542% identity in 96 aa overlap (1-95:1-288)
10 20 30 40 50
HD37L DILITQSPKSMSMSVGERVTLTCKASENV-VTYVSWYQQKPEQSPKLLIYGASNRYTGVP
.:..:::: ..:.: :::.::.:.::..: .:.::::::: :.:.:::::::.: ::.:
V268 EIVMTQSPATLSLSPGERATLSCRASQSVSSSYLSWYQQKPGQAPRLLIYGASTRATGIP
10 40 70 100 130 160
60 70 80 90 100
HD37L DRFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: : :. :
V268 ARFSGSGSGTDFTLTISSLQPEDFAVYYCQQDYNLP
190 220 250 280
>>DPK23_L25- (290 aa)
Frame: 1 initn: 344 init1: 262 opt: 420 Z-score: 79.2 expect() 8.4
63.542% identity in 96 aa overlap (1-95:1-288)
10 20 30 40 50
HD37L DILITQSPKSMSMSVGERVTLTCKASENV-VTYVSWYQQKPEQSPKLLIYGASNRYTGVP
.:..:::: ..:.: :::.::.:.::..: .:.::::::: :.:.:::::::.: ::.:
DPK23_ EIVMTQSPATLSLSPGERATLSCRASQSVSSSYLSWYQQKPGQAPRLLIYGASTRATGIP
10 40 70 100 130 160
60 70 80 90 100
HD37L DRFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: : :. :
DPK23_ ARFSGSGSGTDFTLTISSLQPEDFAVYYCQQDYNLP
190 220 250 280
>>DPK6_VB (287 aa)
Frame: 1 initn: 369 init1: 369 opt: 419 Z-score: 79.2 expect() 8.4
62.105% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::.:.: :::.:::.::.::... ....:::::: ..::::::.::. .:::.
DPK6_V DIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: :. :.:
DPK6_V RFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFP
190 220 250 280
>>VB (287 aa)
Frame: 1 initn: 369 init1: 369 opt: 419 Z-score: 79.2 expect() 8.4
62.105% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::.:.: :::.:::.::.::... ....:::::: ..::::::.::. .:::.
VB DIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: :. :.:
VB RFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFP
190 220 250 280
>>DPK5_VB (287 aa)
Frame: 1 initn: 369 init1: 369 opt: 419 Z-score: 79.2 expect() 8.4
62.105% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::.:.: :::.:::.::.::... ....:::::: ..::::::.::. .:::.
DPK5_V DIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: :. :.:
DPK5_V RFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFP
190 220 250 280
>>DPK1_O18- (287 aa)
Frame: 1 initn: 379 init1: 379 opt: 417 Z-score: 79.0 expect() 8.7
63.158% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::.:.: :::.:::.::.::... .:..:::::: ..:::::: ::: ::::.
DPK1_O DIQMTQSPSSLSASVGDRVTITCQASQDISNYLNWYQQKPGKAPKLLIYDASNLETGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.::::.::::.: ::.: :.: : . :
DPK1_O RFSGSGSGTDFTFTISSLQPEDIATYYCQQYDNLP
190 220 250 280
>>HK101_HK146- (287 aa)
Frame: 1 initn: 417 init1: 417 opt: 417 Z-score: 79.0 expect() 8.7
62.105% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::.:.: :::.:::.::.: ... ....:::::::..:: :::.::. .:::.
HK101_ DIQMTQSPSSLSASVGDRVTITCRARQGISSWLAWYQQKPEKAPKSLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: : :::
HK101_ RFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
190 220 250 280
>>V108 (287 aa)
Frame: 1 initn: 368 init1: 368 opt: 416 Z-score: 78.9 expect() 8.8
63.158% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::.:.: :::.:::.::.::... . .::::::: :.: ::::.::. .:::.
V108 DIQVTQSPSSLSASVGDRVTITCRASQGISNGLSWYQQKPGQAPTLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: : :. :
V108 RFSGSGSGTDFTLTISSLQPEDVATYYCLQDYTTP
190 220 250 280
>>VG_38K- (287 aa)
Frame: 1 initn: 368 init1: 368 opt: 416 Z-score: 78.9 expect() 8.8
61.053% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
.:..:::: ..:.: :::.::.:.::..: .:..:::::: :.:.:::: :::: ::.:
VG_38K EIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPA
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.. ::.: :.: : ..:
VG_38K RFSGSGSGTDFTLTISSLEPEDFAVYYCQQRSNWP
190 220 250 280
>>VE (287 aa)
Frame: 1 initn: 412 init1: 412 opt: 416 Z-score: 78.9 expect() 8.8
62.766% identity in 94 aa overlap (2-95:4-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
: .::::.:.: :.:.:::.::.::... .:..:::::: ..::::::.::. .:::.
VE AIRMTQSPSSFSASTGDRVTITCRASQGISSYLAWYQQKPGKAPKLLIYAASTLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::: .:.::.: :.: : ::::
VE RFSGSGSGTDFTLTISCLQSEDFATYYCQQYYSYP
190 220 250 280
>>HK137 (287 aa)
Frame: 1 initn: 414 init1: 414 opt: 414 Z-score: 78.7 expect() 9
62.105% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::.:.: :::.:::.::.::... .:..:.:::: ..:: :::.::. .:::.
HK137 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWFQQKPGKAPKSLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: : :::
HK137 RFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
190 220 250 280
>>DPK8_VD (287 aa)
Frame: 1 initn: 412 init1: 412 opt: 412 Z-score: 78.5 expect() 9.2
62.105% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::. .: :::.:::.::.::... .:..:::::: ..::::::.::. .:::.
DPK8_V DIQLTQSPSFLSASVGDRVTITCRASQGISSYLAWYQQKPGKAPKLLIYAASTLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:.:::::::.: ::.: :.: : :::
DPK8_V RFSGSGSGTEFTLTISSLQPEDFATYYCQQLNSYP
190 220 250 280
>>DPK3_L11 (287 aa)
Frame: 1 initn: 406 init1: 406 opt: 411 Z-score: 78.4 expect() 9.3
62.766% identity in 94 aa overlap (2-95:4-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
: .::::.:.: :::.:::.::.::... . ..:::::: ..::::::.::. .:::.
DPK3_L AIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: : :.::
DPK3_L RFSGSGSGTDFTLTISSLQPEDFATYYCLQDYNYP
190 220 250 280
>>DPK24_VKIVKLOB- (305 aa)
Frame: 1 initn: 388 init1: 301 opt: 413 Z-score: 78.4 expect() 9.3
62.376% identity in 101 aa overlap (1-95:1-303)
10 20 30 40 50
HD37L DILITQSPKSMSMSVGERVTLTCKASENVV------TYVSWYQQKPEQSPKLLIYGASNR
::..:::: :...:.:::.:..::.:..:. .:..:::::: : :::::: ::.:
DPK24_ DIVMTQSPDSLAVSLGERATINCKSSQSVLYSSNNKNYLAWYQQKPGQPPKLLIYWASTR
10 40 70 100 130 160
60 70 80 90 100
HD37L YTGVPDRFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
.::::::.::::.:::::::::.::::.: :.: : :: :
DPK24_ ESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSTP
190 220 250 280
>>LFVK431 (287 aa)
Frame: 1 initn: 410 init1: 410 opt: 410 Z-score: 78.3 expect() 9.4
61.053% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
HD37L DILITQSPKSMSMSVGERVTLTCKASENVVTYVSWYQQKPEQSPKLLIYGASNRYTGVPD
:: .::::.:.: :::.:::.::.::... .:..:.:::: ..:: :::.::. .:::.
LFVK43 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWFQQKPGKAPKSLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
HD37L RFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
.:.::::.:::::::::.: ::.: :.: : :::
LFVK43 KFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
190 220 250 280
>>VH (290 aa)
Frame: 1 initn: 336 init1: 249 opt: 408 Z-score: 78.1 expect() 9.7
61.458% identity in 96 aa overlap (1-95:1-288)
10 20 30 40 50
HD37L DILITQSPKSMSMSVGERVTLTCKASENV-VTYVSWYQQKPEQSPKLLIYGASNRYTGVP
.:..:::: ..:.: ::::::.:.::..: .:..:::::: :.:.:::::::.: :..:
VH EIVMTQSPPTLSLSPGERVTLSCRASQSVSSSYLTWYQQKPGQAPRLLIYGASTRATSIP
10 40 70 100 130 160
60 70 80 90 100
HD37L DRFTGSGSATDFTLTISSVQAEDLADYHCGQGYSYPYTFGGGTKLEIKR
::.::::.:::::::::.: ::.: :.: : .. :
VH ARFSGSGSGTDFTLTISSLQPEDFAVYYCQQDHNLP
190 220 250 280
108 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Fri Jul 21 15:37:24 2000 done: Fri Jul 21 15:37:25 2000
Scan time: 0.250 Display time: 0.040
Function used was TFASTA
Date: Fri, 21 Jul 2000 15:42:05 +0100
Subject: FASTA results: HD37LJ
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-1156404: 13 aa
>HD37LJ
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 2.7859+/-0.00771; mu= 4.0855+/- 0.433;
mean_var=14.2928+/-11.555, 0's: 11 Z-trim: 9 B-trim: 5 in 1/20
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.140
The best scores are: initn init1 opt z-sc E(618)
JK2 ( 39) [3] 68 68 68 192.1 4.2e-06
JK4 ( 38) [2] 66 66 66 187.0 8.2e-06
JK1 ( 38) [2] 56 56 58 165.8 0.00012
JK5 ( 38) [2] 57 57 57 163.2 0.00017
JK3 ( 38) [2] 52 52 56 160.5 0.00024
>>JK2 (39 aa)
Frame: 3 initn: 68 init1: 68 opt: 68 Z-score: 192.1 expect() 4.2e-06
91.667% identity in 12 aa overlap (1-12:3-38)
10
HD37LJ YTFGGGTKLEIKR
:::: :::::::
JK2 YTFGQGTKLEIK
30
>>JK4 (38 aa)
Frame: 2 initn: 66 init1: 66 opt: 66 Z-score: 187.0 expect() 8.2e-06
90.909% identity in 11 aa overlap (2-12:5-37)
10
HD37LJ YTFGGGTKLEIKR
:::::::.:::
JK4 LTFGGGTKVEIK
20
>>JK1 (38 aa)
Frame: 2 initn: 56 init1: 56 opt: 58 Z-score: 165.8 expect() 0.00012
75.000% identity in 12 aa overlap (1-12:2-37)
10
HD37LJ YTFGGGTKLEIKR
.::: :::.:::
JK1 WTFGQGTKVEIK
20
>>JK5 (38 aa)
Frame: 2 initn: 57 init1: 57 opt: 57 Z-score: 163.2 expect() 0.00017
81.818% identity in 11 aa overlap (2-12:5-37)
10
HD37LJ YTFGGGTKLEIKR
::: ::.::::
JK5 ITFGQGTRLEIK
20
>>JK3 (38 aa)
Frame: 2 initn: 52 init1: 52 opt: 56 Z-score: 160.5 expect() 0.00024
66.667% identity in 12 aa overlap (1-12:2-37)
10
HD37LJ YTFGGGTKLEIKR
.::: :::..::
JK3 FTFGPGTKVDIK
20
13 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Fri Jul 21 15:42:05 2000 done: Fri Jul 21 15:42:05 2000
Scan time: 0.140 Display time: 0.010
Function used was TFASTA
Mike Clark