Mike Clark
"An antibody engineer who also enjoys the mountains."
mrc7@cam.ac.uk
Mike's home-page
© Mike Clark, PhD., Cambridge University, UK
Last updated on 16th March 2000
ID HSFOG1H standard; RNA; HUM; 429 BP.
XX
AC X64150;
XX
SV X64150.1
XX
DT 06-MAR-1993 (Rel. 35, Created)
DT 26-JUL-1997 (Rel. 52, Last updated, Version 6)
XX
DE H.sapiens mRNA for Fog1H heavy chain variable Ig domain
XX
KW heavy chain; Ig heavy chain; immunoglobulin heavy chain variable region;
KW variable region.
XX
OS Homo sapiens (human)
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia; Eutheria;
OC Primates; Catarrhini; Hominidae; Homo.
XX
RN [1]
RP 1-429
RA Hughes-Jones N.C.;
RT ;
RL Submitted (08-JAN-1992) to the EMBL/GenBank/DDBJ databases.
RL N.C. Hughes-Jones, Inst of Animal Physiology and Genetics Research,
RL Babraham Hall, Cambridge CB2 4AT, UK
XX
RN [2]
RP 1-57, 58-429
RX MEDLINE; 93107334.
RA Bye J.M., Carter C., Cui Y., Gorick B.D., Songsivilai S., Winter G.,
RA Hughes-Jones N.C., Marks J.D.;
RT "Germline variable region gene segment derivation of human monoclonal
RT anti-Rh(D) antibodies. Evidence for affinity maturation by somatic
RT hypermutation and repertoire shift";
RL J. Clin. Invest. 90:2481-2490(1992).
XX
DR IMGT/LIGM; X64150; Release 98.12.
XX
CC See also X64148-69
XX
FH Key Location/Qualifiers
FH
FT source 1..429
FT /chromosome="14"
FT /db_xref="taxon:9606"
FT /organism="Homo sapiens"
FT /cell_type="B-lymphocytes"
FT /cell_line="Fog-1"
FT /isolate="blood donor Fo"
FT misc_feature 1..57
FT /note="leader sequence"
FT misc_feature 58..429
FT /note="VDJ region"
FT misc_feature 58..348
FT /note="Vh region"
FT misc_feature 148..162
FT /note="CDR1 region"
FT misc_feature 205..252
FT /note="CDR2 region"
FT misc_feature 349..374
FT /note="D region"
FT misc_feature 349..396
FT /note="CDR3 region"
FT misc_feature 375..429
FT /note="J region"
XX
SQ Sequence 429 BP; 83 A; 130 C; 121 G; 95 T; 0 other;
atgaaacacc tgtggttcgt cctcctcctg gtggcagctc ccagatgggt cctgtcccag 60
gtgcatctac agcagtgggg cacagggctg ttgaagcctt cggagaccct gtccctcacc 120
tgcgctgtcc atggtgggtc cttcaatgtt tactactgga cctggatccg ccagccccca 180
ggaaaggcgc tggagtggat tggggaaatc aatcatagtg gaggcgccaa ctacaatccg 240
tccctcaaga gtcgagtcac catgtcagca gacacgtcca agaaccagtt ctccctgaaa 300
ctgacctctg tgaccgccgc ggacacggct gtgttttatt gtgcgagagg ccggtcccgt 360
tatagtggtt acggcttcta ctccggcatg gacgtctggg gcccagggac cacggtcacc 420
gtctcctca 429
//
ID HSFOG1L standard; RNA; HUM; 390 BP.
XX
AC X64163;
XX
SV X64163.1
XX
DT 06-MAR-1993 (Rel. 35, Created)
DT 26-JUL-1997 (Rel. 52, Last updated, Version 7)
XX
DE H.sapiens mRNA for Fog1L kappa light chain variable Ig domain
XX
KW Ig kappa light chain; Ig light chain;
KW immunoglobulin light chain variable region; light chain; variable region.
XX
OS Homo sapiens (human)
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia; Eutheria;
OC Primates; Catarrhini; Hominidae; Homo.
XX
RN [1]
RP 1-390
RA Hughes-Jones N.C.;
RT ;
RL Submitted (08-JAN-1992) to the EMBL/GenBank/DDBJ databases.
RL N.C. Hughes-Jones, Inst of Animal Physiology and Genetics Research,
RL Babraham Hall, Cambridge CB2 4AT, UK
XX
RN [2]
RP 1-66, 67-390
RX MEDLINE; 93107334.
RA Bye J.M., Carter C., Cui Y., Gorick B.D., Songsivilai S., Winter G.,
RA Hughes-Jones N.C., Marks J.D.;
RT "Germline variable region gene segment derivation of human monoclonal
RT anti-Rh(D) antibodies. Evidence for affinity maturation by somatic
RT hypermutation and repertoire shift";
RL J. Clin. Invest. 90:2481-2490(1992).
XX
DR IMGT/LIGM; X64163; Release 98.12.
XX
CC See also X64148-69
XX
FH Key Location/Qualifiers
FH
FT source 1..390
FT /chromosome="2"
FT /db_xref="taxon:9606"
FT /organism="Homo sapiens"
FT /cell_type="B-lymphocytes"
FT /cell_line="Fog-1"
FT /isolate="blood donor Fo"
FT misc_feature 1..66
FT /note="leader sequence"
FT misc_feature 67..390
FT /note="VJ region"
FT misc_feature 67..351
FT /note="V kappa region"
FT misc_feature 136..168
FT /note="CDR1 region"
FT misc_feature 214..234
FT /note="CDR2 region"
FT misc_feature 331..357
FT /note="CDR3 region"
FT misc_feature 352..390
FT /note="J region"
XX
SQ Sequence 390 BP; 90 A; 105 C; 99 G; 96 T; 0 other;
atggacatga gggtccccgc tcagctcctg gggctcctgc tgctctggtt cccaggtgcc 60
aggtgtgaca tccagatgac ccagtctcca tcctccctgt ctgcatctgt aggagacaga 120
gtcaccatca cttgccgggc aagtcaggtc attagaaatg atttggtctg gtttcagcag 180
aaaccaggga tagcccctaa gcgcctgatc tatgctgcat ccagtttgca aagtggggtc 240
ccatcaaggt tcagtggcag tggatttggg acagaattca ctctcacaat cagcagcctg 300
cagcctgaag attttgcaac ttattactgt ctacagcatt tcagttaccc gtacactttt 360
ggccagggga ccaagctgga gatcaaacga 390
//
Date: Thu, 16 Mar 2000 14:01:03 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-6139562: 124 aa
>fog1vh
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 53.0747+/-0.0113; mu= -150.9810+/- 0.490;
mean_var=11730.4478+/-9964.863, 0's: 1 Z-trim: 0 B-trim: 0 in 0/21
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.290
The best scores are: initn init1 opt z-sc E(637)
V4-34 ( 293) [1] 588 588 588 90.4 2
VH5_4D76- ( 293) [1] 588 588 588 90.4 2
DP-63_VH4-21 ( 293) [1] 588 588 588 90.4 2
H3 ( 288) [1] 578 578 578 89.6 2.2
TOU-VH4-21 ( 291) [1] 578 578 578 89.5 2.3
4-44 ( 293) [1] 573 573 573 89.0 2.4
4-443 ( 293) [1] 556 556 556 87.4 2.9
4-37 ( 293) [1] 556 556 556 87.4 2.9
4-36 ( 293) [1] 555 555 555 87.3 3
4-38 ( 293) [1] 549 549 549 86.8 3.2
>>V4-34 (293 aa)
Frame: 1 initn: 588 init1: 588 opt: 588 Z-score: 90.4 expect() 2
86.598% identity in 97 aa overlap (1-97:1-291)
10 20 30 40 50 60
fog1vh QVHLQQWGTGLLKPSETLSLTCAVHGGSFNVYYWTWIRQPPGKALEWIGEINHSGGANYN
::.:::::.:::::::::::::::.::::. :::.::::::::.:::::::::::..:::
V4-34 QVQLQQWGAGLLKPSETLSLTCAVYGGSFSGYYWSWIRQPPGKGLEWIGEINHSGSTNYN
10 40 70 100 130 160
70 80 90 100 110 120
fog1vh PSLKSRVTMSADTSKNQFSLKLTSVTAADTAVFYCARGRSRYSGYGFYSGMDVWGPGTTV
::::::::.:.:::::::::::.:::::::::.::::
V4-34 PSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCAR
190 220 250 280
fog1vh TVSS
>>VH5_4D76- (293 aa)
Frame: 1 initn: 588 init1: 588 opt: 588 Z-score: 90.4 expect() 2
86.598% identity in 97 aa overlap (1-97:1-291)
10 20 30 40 50 60
fog1vh QVHLQQWGTGLLKPSETLSLTCAVHGGSFNVYYWTWIRQPPGKALEWIGEINHSGGANYN
::.:::::.:::::::::::::::.::::. :::.::::::::.:::::::::::..:::
VH5_4D QVQLQQWGAGLLKPSETLSLTCAVYGGSFSGYYWSWIRQPPGKGLEWIGEINHSGSTNYN
10 40 70 100 130 160
70 80 90 100 110 120
fog1vh PSLKSRVTMSADTSKNQFSLKLTSVTAADTAVFYCARGRSRYSGYGFYSGMDVWGPGTTV
::::::::.:.:::::::::::.:::::::::.::::
VH5_4D PSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCAR
190 220 250 280
fog1vh TVSS
>>DP-63_VH4-21 (293 aa)
Frame: 1 initn: 588 init1: 588 opt: 588 Z-score: 90.4 expect() 2
86.598% identity in 97 aa overlap (1-97:1-291)
10 20 30 40 50 60
fog1vh QVHLQQWGTGLLKPSETLSLTCAVHGGSFNVYYWTWIRQPPGKALEWIGEINHSGGANYN
::.:::::.:::::::::::::::.::::. :::.::::::::.:::::::::::..:::
DP-63_ QVQLQQWGAGLLKPSETLSLTCAVYGGSFSGYYWSWIRQPPGKGLEWIGEINHSGSTNYN
10 40 70 100 130 160
70 80 90 100 110 120
fog1vh PSLKSRVTMSADTSKNQFSLKLTSVTAADTAVFYCARGRSRYSGYGFYSGMDVWGPGTTV
::::::::.:.:::::::::::.:::::::::.::::
DP-63_ PSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCAR
190 220 250 280
fog1vh TVSS
>>H3 (288 aa)
Frame: 1 initn: 578 init1: 578 opt: 578 Z-score: 89.6 expect() 2.2
85.417% identity in 96 aa overlap (1-96:1-288)
10 20 30 40 50 60
fog1vh QVHLQQWGTGLLKPSETLSLTCAVHGGSFNVYYWTWIRQPPGKALEWIGEINHSGGANYN
::.:::::.:::::::::::::::.::.:. :::.::::::::.:::::::::::..:::
H3 QVQLQQWGAGLLKPSETLSLTCAVYGGTFSGYYWSWIRQPPGKGLEWIGEINHSGSTNYN
10 40 70 100 130 160
70 80 90 100 110 120
fog1vh PSLKSRVTMSADTSKNQFSLKLTSVTAADTAVFYCARGRSRYSGYGFYSGMDVWGPGTTV
::::::::.:.:::::::::::.:::::::::.:::
H3 PSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCA
190 220 250 280
fog1vh TVSS
>>TOU-VH4-21 (291 aa)
Frame: 1 initn: 578 init1: 578 opt: 578 Z-score: 89.5 expect() 2.3
85.567% identity in 97 aa overlap (1-97:1-291)
10 20 30 40 50 60
fog1vh QVHLQQWGTGLLKPSETLSLTCAVHGGSFNVYYWTWIRQPPGKALEWIGEINHSGGANYN
::.:::::.:::::::::::::::.::::. :::.::::::::.::::::: :::..:::
TOU-VH QVQLQQWGAGLLKPSETLSLTCAVYGGSFSGYYWSWIRQPPGKGLEWIGEIIHSGSTNYN
10 40 70 100 130 160
70 80 90 100 110 120
fog1vh PSLKSRVTMSADTSKNQFSLKLTSVTAADTAVFYCARGRSRYSGYGFYSGMDVWGPGTTV
::::::::.:.:::::::::::.:::::::::.::::
TOU-VH PSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCAR
190 220 250 280
fog1vh TVSS
>>4-44 (293 aa)
Frame: 1 initn: 573 init1: 573 opt: 573 Z-score: 89.0 expect() 2.4
84.536% identity in 97 aa overlap (1-97:1-291)
10 20 30 40 50 60
fog1vh QVHLQQWGTGLLKPSETLSLTCAVHGGSFNVYYWTWIRQPPGKALEWIGEINHSGGANYN
::.:::::.:::::::::::::::.::::. :::.::::::::.:::::::::::..: :
4-44 QVQLQQWGAGLLKPSETLSLTCAVYGGSFSGYYWSWIRQPPGKGLEWIGEINHSGSTNNN
10 40 70 100 130 160
70 80 90 100 110 120
fog1vh PSLKSRVTMSADTSKNQFSLKLTSVTAADTAVFYCARGRSRYSGYGFYSGMDVWGPGTTV
::::::.:.:.:::::::::::.:::::::::.::::
4-44 PSLKSRATISVDTSKNQFSLKLSSVTAADTAVYYCAR
190 220 250 280
fog1vh TVSS
>>4-443 (293 aa)
Frame: 1 initn: 556 init1: 556 opt: 556 Z-score: 87.4 expect() 2.9
83.505% identity in 97 aa overlap (1-97:1-291)
10 20 30 40 50 60
fog1vh QVHLQQWGTGLLKPSETLSLTCAVHGGSFNVYYWTWIRQPPGKALEWIGEINHSGGANYN
::.:::::.:::::::::::::::.::::. ::: ::::: ::.:::::::::::..: :
4-443 QVQLQQWGAGLLKPSETLSLTCAVYGGSFSGYYWCWIRQPLGKGLEWIGEINHSGSTNNN
10 40 70 100 130 160
70 80 90 100 110 120
fog1vh PSLKSRVTMSADTSKNQFSLKLTSVTAADTAVFYCARGRSRYSGYGFYSGMDVWGPGTTV
::::::.:.:.:::::::::::.:::::::::.::::
4-443 PSLKSRATISVDTSKNQFSLKLSSVTAADTAVYYCAR
190 220 250 280
fog1vh TVSS
>>4-37 (293 aa)
Frame: 1 initn: 556 init1: 556 opt: 556 Z-score: 87.4 expect() 2.9
82.474% identity in 97 aa overlap (1-97:1-291)
10 20 30 40 50 60
fog1vh QVHLQQWGTGLLKPSETLSLTCAVHGGSFNVYYWTWIRQPPGKALEWIGEINHSGGANYN
::.::. : ::.::::::::::::.::::. :::.::::::::.:::::::::::..:::
4-37 QVQLQESGPGLVKPSETLSLTCAVYGGSFSGYYWSWIRQPPGKGLEWIGEINHSGSTNYN
10 40 70 100 130 160
70 80 90 100 110 120
fog1vh PSLKSRVTMSADTSKNQFSLKLTSVTAADTAVFYCARGRSRYSGYGFYSGMDVWGPGTTV
::::::.:::.::::::: :::.:::::::::.::::
4-37 PSLKSRITMSVDTSKNQFYLKLSSVTAADTAVYYCAR
190 220 250 280
fog1vh TVSS
>>4-36 (293 aa)
Frame: 1 initn: 555 init1: 555 opt: 555 Z-score: 87.3 expect() 3
82.474% identity in 97 aa overlap (1-97:1-291)
10 20 30 40 50 60
fog1vh QVHLQQWGTGLLKPSETLSLTCAVHGGSFNVYYWTWIRQPPGKALEWIGEINHSGGANYN
::.::. : ::.:::.::::::::.::::. :::.::::::::.:::::::::::..:::
4-36 QVQLQESGPGLVKPSQTLSLTCAVYGGSFSGYYWSWIRQPPGKGLEWIGEINHSGSTNYN
10 40 70 100 130 160
70 80 90 100 110 120
fog1vh PSLKSRVTMSADTSKNQFSLKLTSVTAADTAVFYCARGRSRYSGYGFYSGMDVWGPGTTV
::::::::.:.:::::::::::.:::::::::.::::
4-36 PSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCAR
190 220 250 280
fog1vh TVSS
>>4-38 (293 aa)
Frame: 1 initn: 549 init1: 549 opt: 549 Z-score: 86.8 expect() 3.2
82.474% identity in 97 aa overlap (1-97:1-291)
10 20 30 40 50 60
fog1vh QVHLQQWGTGLLKPSETLSLTCAVHGGSFNVYYWTWIRQPPGKALEWIGEINHSGGANYN
::.:::::.:::::::::::::::.:::.. :::.::::: ::.:::::.: ::..:::
4-38 QVQLQQWGAGLLKPSETLSLTCAVYGGSISSYYWSWIRQPAGKGLEWIGRIYTSGSTNYN
10 40 70 100 130 160
70 80 90 100 110 120
fog1vh PSLKSRVTMSADTSKNQFSLKLTSVTAADTAVFYCARGRSRYSGYGFYSGMDVWGPGTTV
::::::::::.:::::::::::.:::::::::.::::
4-38 PSLKSRVTMSVDTSKNQFSLKLSSVTAADTAVYYCAR
190 220 250 280
fog1vh TVSS
124 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Thu Mar 16 14:01:02 2000 done: Thu Mar 16 14:01:03 2000
Scan time: 0.290 Display time: 0.030
Function used was TFASTA
Date: Thu, 16 Mar 2000 19:25:08 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-6044182: 27 aa
>fog1vhj
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 5.2608+/-0.00745; mu= 0.9810+/- 0.419;
mean_var=65.2981+/-44.752, 0's: 1 Z-trim: 3 B-trim: 8 in 1/19
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.170
The best scores are: initn init1 opt z-sc E(634)
JH6A ( 63) [3] 93 93 108 155.5 0.00048
JH6B ( 63) [3] 93 93 108 155.5 0.00048
JH6C ( 63) [3] 85 85 97 141.9 0.0027
JH3A ( 50) [2] 72 72 72 112.4 0.12
JH3B ( 50) [2] 59 59 71 111.2 0.14
JH2 ( 53) [2] 57 57 67 105.9 0.28
JH4A ( 48) [3] 59 59 66 105.3 0.3
JH4B ( 48) [3] 59 59 66 105.3 0.3
JH4D ( 48) [3] 59 59 66 105.3 0.3
JH5A ( 51) [3] 59 59 65 103.6 0.37
>>JH6A (63 aa)
Frame: 3 initn: 93 init1: 93 opt: 108 Z-score: 155.5 expect() 0.00048
80.000% identity in 20 aa overlap (8-27:3-62)
10 20
fog1vh GRSRYSGYGFYSGMDVWGPGTTVTVSS
: .: :::::: ::::::::
JH6A YYYYYGMDVWGQGTTVTVSS
30 60
>>JH6B (63 aa)
Frame: 3 initn: 93 init1: 93 opt: 108 Z-score: 155.5 expect() 0.00048
80.000% identity in 20 aa overlap (8-27:3-62)
10 20
fog1vh GRSRYSGYGFYSGMDVWGPGTTVTVSS
: .: :::::: ::::::::
JH6B YYYYYGMDVWGQGTTVTVSS
30 60
>>JH6C (63 aa)
Frame: 3 initn: 85 init1: 85 opt: 97 Z-score: 141.9 expect() 0.0027
75.000% identity in 20 aa overlap (8-27:3-62)
10 20
fog1vh GRSRYSGYGFYSGMDVWGPGTTVTVSS
: .: ::::: ::::::::
JH6C YYYYYYMDVWGKGTTVTVSS
30 60
>>JH3A (50 aa)
Frame: 2 initn: 72 init1: 72 opt: 72 Z-score: 112.4 expect() 0.12
84.615% identity in 13 aa overlap (15-27:11-49)
10 20
fog1vh GRSRYSGYGFYSGMDVWGPGTTVTVSS
:::: :: :::::
JH3A DAFDVWGQGTMVTVSS
20
>>JH3B (50 aa)
Frame: 2 initn: 59 init1: 59 opt: 71 Z-score: 111.2 expect() 0.14
76.923% identity in 13 aa overlap (15-27:11-49)
10 20
fog1vh GRSRYSGYGFYSGMDVWGPGTTVTVSS
:.:: :: :::::
JH3B DAFDIWGQGTMVTVSS
20
>>JH2 (53 aa)
Frame: 2 initn: 57 init1: 57 opt: 67 Z-score: 105.9 expect() 0.28
64.706% identity in 17 aa overlap (11-27:2-52)
10 20
fog1vh GRSRYSGYGFYSGMDVWGPGTTVTVSS
: .:.:: :: :::::
JH2 YWYFDLWGRGTLVTVSS
20 50
>>JH4A (48 aa)
Frame: 3 initn: 59 init1: 59 opt: 66 Z-score: 105.3 expect() 0.3
76.923% identity in 13 aa overlap (15-27:9-47)
10 20
fog1vh GRSRYSGYGFYSGMDVWGPGTTVTVSS
: :: :: :::::
JH4A YFDYWGQGTLVTVSS
30
>>JH4B (48 aa)
Frame: 3 initn: 59 init1: 59 opt: 66 Z-score: 105.3 expect() 0.3
76.923% identity in 13 aa overlap (15-27:9-47)
10 20
fog1vh GRSRYSGYGFYSGMDVWGPGTTVTVSS
: :: :: :::::
JH4B YFDYWGQGTLVTVSS
30
>>JH4D (48 aa)
Frame: 3 initn: 59 init1: 59 opt: 66 Z-score: 105.3 expect() 0.3
76.923% identity in 13 aa overlap (15-27:9-47)
10 20
fog1vh GRSRYSGYGFYSGMDVWGPGTTVTVSS
: :: :: :::::
JH4D YFDYWGQGTLVTVSS
30
>>JH5A (51 aa)
Frame: 3 initn: 59 init1: 59 opt: 65 Z-score: 103.6 expect() 0.37
76.923% identity in 13 aa overlap (15-27:12-50)
10 20
fog1vh GRSRYSGYGFYSGMDVWGPGTTVTVSS
: :: :: :::::
JH5A NWFDSWGQGTLVTVSS
30
27 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Thu Mar 16 19:25:08 2000 done: Thu Mar 16 19:25:08 2000
Scan time: 0.170 Display time: 0.020
Function used was TFASTA
Date: Thu, 16 Mar 2000 12:54:07 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-6161380: 108 aa
>fog1vk
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 44.9543+/-0.0108; mu= -133.1882+/- 0.495;
mean_var=8449.8381+/-7150.965, 0's: 1 Z-trim: 0 B-trim: 0 in 0/21
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.300
The best scores are: initn init1 opt z-sc E(637)
A30_SG3 ( 287) [1] 577 577 577 99.6 0.62
DPK3_L11 ( 287) [1] 548 548 548 96.4 0.93
HK137 ( 287) [1] 539 539 539 95.4 1.1
DPK2_L14 ( 287) [1] 534 534 536 95.1 1.1
LFVK431 ( 287) [1] 535 535 535 95.0 1.1
DPK9_O12- ( 287) [1] 500 500 521 93.5 1.4
DPK7_HK134- ( 287) [1] 516 516 516 92.9 1.4
DPK6_VB ( 287) [1] 495 495 513 92.6 1.5
VB ( 287) [1] 495 495 513 92.6 1.5
DPK5_VB ( 287) [1] 495 495 513 92.6 1.5
>>A30_SG3 (287 aa)
Frame: 1 initn: 577 init1: 577 opt: 577 Z-score: 99.6 expect() 0.62
93.684% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
fog1vk DIQMTQSPSSLSASVGDRVTITCRASQVIRNDLVWFQQKPGIAPKRLIYAASSLQSGVPS
::::::::::::::::::::::::::: ::::: :.::::: ::::::::::::::::::
A30_SG DIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKRLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
fog1vk RFSGSGFGTEFTLTISSLQPEDFATYYCLQHFSYPYTFGQGTKLEIKR
:::::: :::::::::::::::::::::::: :::
A30_SG RFSGSGSGTEFTLTISSLQPEDFATYYCLQHNSYP
190 220 250 280
>>DPK3_L11 (287 aa)
Frame: 1 initn: 548 init1: 548 opt: 548 Z-score: 96.4 expect() 0.93
89.362% identity in 94 aa overlap (2-95:4-285)
10 20 30 40 50 60
fog1vk DIQMTQSPSSLSASVGDRVTITCRASQVIRNDLVWFQQKPGIAPKRLIYAASSLQSGVPS
:::::::::::::::::::::::::: ::::: :.::::: ::: ::::::::::::::
DPK3_L AIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
fog1vk RFSGSGFGTEFTLTISSLQPEDFATYYCLQHFSYPYTFGQGTKLEIKR
:::::: ::.:::::::::::::::::::: ..::
DPK3_L RFSGSGSGTDFTLTISSLQPEDFATYYCLQDYNYP
190 220 250 280
>>HK137 (287 aa)
Frame: 1 initn: 539 init1: 539 opt: 539 Z-score: 95.4 expect() 1.1
88.421% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
fog1vk DIQMTQSPSSLSASVGDRVTITCRASQVIRNDLVWFQQKPGIAPKRLIYAASSLQSGVPS
::::::::::::::::::::::::::: : : :.::::::: ::: ::::::::::::::
HK137 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWFQQKPGKAPKSLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
fog1vk RFSGSGFGTEFTLTISSLQPEDFATYYCLQHFSYPYTFGQGTKLEIKR
:::::: ::.:::::::::::::::::: :. :::
HK137 RFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
190 220 250 280
>>DPK2_L14 (287 aa)
Frame: 1 initn: 534 init1: 534 opt: 536 Z-score: 95.1 expect() 1.1
86.316% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
fog1vk DIQMTQSPSSLSASVGDRVTITCRASQVIRNDLVWFQQKPGIAPKRLIYAASSLQSGVPS
.::::::::..:::::::::::::: : : : :.::::::: .::.::::::::::::::
DPK2_L NIQMTQSPSAMSASVGDRVTITCRARQGISNYLAWFQQKPGKVPKHLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
fog1vk RFSGSGFGTEFTLTISSLQPEDFATYYCLQHFSYPYTFGQGTKLEIKR
:::::: :::::::::::::::::::::::: :::
DPK2_L RFSGSGSGTEFTLTISSLQPEDFATYYCLQHNSYP
190 220 250 280
>>LFVK431 (287 aa)
Frame: 1 initn: 535 init1: 535 opt: 535 Z-score: 95.0 expect() 1.1
87.368% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
fog1vk DIQMTQSPSSLSASVGDRVTITCRASQVIRNDLVWFQQKPGIAPKRLIYAASSLQSGVPS
::::::::::::::::::::::::::: : : :.::::::: ::: ::::::::::::::
LFVK43 DIQMTQSPSSLSASVGDRVTITCRASQGISNYLAWFQQKPGKAPKSLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
fog1vk RFSGSGFGTEFTLTISSLQPEDFATYYCLQHFSYPYTFGQGTKLEIKR
.::::: ::.:::::::::::::::::: :. :::
LFVK43 KFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
190 220 250 280
>>DPK9_O12- (287 aa)
Frame: 1 initn: 500 init1: 500 opt: 521 Z-score: 93.5 expect() 1.4
85.263% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
fog1vk DIQMTQSPSSLSASVGDRVTITCRASQVIRNDLVWFQQKPGIAPKRLIYAASSLQSGVPS
::::::::::::::::::::::::::: : . : :.::::: ::: ::::::::::::::
DPK9_O DIQMTQSPSSLSASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
fog1vk RFSGSGFGTEFTLTISSLQPEDFATYYCLQHFSYPYTFGQGTKLEIKR
:::::: ::.:::::::::::::::::: : .: :
DPK9_O RFSGSGSGTDFTLTISSLQPEDFATYYCQQSYSTP
190 220 250 280
>>DPK7_HK134- (287 aa)
Frame: 1 initn: 516 init1: 516 opt: 516 Z-score: 92.9 expect() 1.4
85.263% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
fog1vk DIQMTQSPSSLSASVGDRVTITCRASQVIRNDLVWFQQKPGIAPKRLIYAASSLQSGVPS
::::::::::::::::::::::::::: : . :.:.:::: ::: ::::::::::::::
DPK7_H DIQMTQSPSSLSASVGDRVTITCRASQGISSWLAWYQQKPEKAPKSLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
fog1vk RFSGSGFGTEFTLTISSLQPEDFATYYCLQHFSYPYTFGQGTKLEIKR
:::::: ::.:::::::::::::::::: :. :::
DPK7_H RFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP
190 220 250 280
>>DPK6_VB (287 aa)
Frame: 1 initn: 495 init1: 495 opt: 513 Z-score: 92.6 expect() 1.5
84.211% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
fog1vk DIQMTQSPSSLSASVGDRVTITCRASQVIRNDLVWFQQKPGIAPKRLIYAASSLQSGVPS
::::::::::.:::::::::::::::: : . :.:.::::: ::: ::::::::::::::
DPK6_V DIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
fog1vk RFSGSGFGTEFTLTISSLQPEDFATYYCLQHFSYPYTFGQGTKLEIKR
:::::: ::.:::::::::::::::::: : :.:
DPK6_V RFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFP
190 220 250 280
>>VB (287 aa)
Frame: 1 initn: 495 init1: 495 opt: 513 Z-score: 92.6 expect() 1.5
84.211% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
fog1vk DIQMTQSPSSLSASVGDRVTITCRASQVIRNDLVWFQQKPGIAPKRLIYAASSLQSGVPS
::::::::::.:::::::::::::::: : . :.:.::::: ::: ::::::::::::::
VB DIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
fog1vk RFSGSGFGTEFTLTISSLQPEDFATYYCLQHFSYPYTFGQGTKLEIKR
:::::: ::.:::::::::::::::::: : :.:
VB RFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFP
190 220 250 280
>>DPK5_VB (287 aa)
Frame: 1 initn: 495 init1: 495 opt: 513 Z-score: 92.6 expect() 1.5
84.211% identity in 95 aa overlap (1-95:1-285)
10 20 30 40 50 60
fog1vk DIQMTQSPSSLSASVGDRVTITCRASQVIRNDLVWFQQKPGIAPKRLIYAASSLQSGVPS
::::::::::.:::::::::::::::: : . :.:.::::: ::: ::::::::::::::
DPK5_V DIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPS
10 40 70 100 130 160
70 80 90 100
fog1vk RFSGSGFGTEFTLTISSLQPEDFATYYCLQHFSYPYTFGQGTKLEIKR
:::::: ::.:::::::::::::::::: : :.:
DPK5_V RFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFP
190 220 250 280
108 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Thu Mar 16 12:54:06 2000 done: Thu Mar 16 12:54:07 2000
Scan time: 0.300 Display time: 0.020
Function used was TFASTA
Date: Thu, 16 Mar 2000 19:22:38 +0000
TFASTA translates and searches a DNA sequence data bank
version 3.2t07 August 17, 1999
Please cite:
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
/tmp/.fasta3-search-6156754: 13 aa
>fog1vkj
vs V-Base too library
searching /data/gcgblast/v-base 0 library
167083 residues in 638 sequences
Expectation_n fit: rho(ln(x))= 1.0442+/-0.00865; mu= 12.9078+/- 0.488;
mean_var=15.6188+/-10.821, 0's: 16 Z-trim: 5 B-trim: 9 in 1/19
TFASTA (3.27 August, 1999) function [optimized, BL50 matrix (15:-5)] ktup: 2
join: 36, opt: 36, gap-pen: -16/ -2, width: 16 reg.-scaled
Scan time: 0.130
The best scores are: initn init1 opt z-sc E(617)
JK2 ( 39) [3] 77 77 77 202.5 1.1e-06
JK1 ( 38) [2] 65 65 67 177.3 2.8e-05
JK5 ( 38) [2] 66 66 66 174.7 3.9e-05
JK3 ( 38) [2] 53 53 57 152.0 0.00073
JK4 ( 38) [2] 56 56 56 149.4 0.001
>>JK2 (39 aa)
Frame: 3 initn: 77 init1: 77 opt: 77 Z-score: 202.5 expect() 1.1e-06
100.000% identity in 12 aa overlap (1-12:3-38)
10
fog1vk YTFGQGTKLEIKR
::::::::::::
JK2 YTFGQGTKLEIK
30
>>JK1 (38 aa)
Frame: 2 initn: 65 init1: 65 opt: 67 Z-score: 177.3 expect() 2.8e-05
83.333% identity in 12 aa overlap (1-12:2-37)
10
fog1vk YTFGQGTKLEIKR
.:::::::.:::
JK1 WTFGQGTKVEIK
20
>>JK5 (38 aa)
Frame: 2 initn: 66 init1: 66 opt: 66 Z-score: 174.7 expect() 3.9e-05
90.909% identity in 11 aa overlap (2-12:5-37)
10
fog1vk YTFGQGTKLEIKR
::::::.::::
JK5 ITFGQGTRLEIK
20
>>JK3 (38 aa)
Frame: 2 initn: 53 init1: 53 opt: 57 Z-score: 152.0 expect() 0.00073
66.667% identity in 12 aa overlap (1-12:2-37)
10
fog1vk YTFGQGTKLEIKR
.::: :::..::
JK3 FTFGPGTKVDIK
20
>>JK4 (38 aa)
Frame: 2 initn: 56 init1: 56 opt: 56 Z-score: 149.4 expect() 0.001
81.818% identity in 11 aa overlap (2-12:5-37)
10
fog1vk YTFGQGTKLEIKR
::: :::.:::
JK4 LTFGGGTKVEIK
20
13 residues in 1 query sequences
167083 residues in 638 library sequences
Tcomplib (4 proc)[version 3.2t07 August 17, 1999]
start: Thu Mar 16 19:22:38 2000 done: Thu Mar 16 19:22:38 2000
Scan time: 0.130 Display time: 0.010
Function used was TFASTA
Mike Clark