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Using CRISPR to identify host factors involved in the replication of viruses

Co-supervisors: Dr Colin Crump and Dr Rupert Beale
Division: Virology
Based at Division of Virology, Addenbrooke’s Hospital
Project title: Using CRISPR to identify host factors involved in the replication of viruses

This project will employ CRISPR/Cas9-based screens to identify host factors that have pro-viral or anti-viral functions. Our laboratories work on influenza and HSV, and we have a shared interest in BK polyomavirus. This small DNA virus encodes only six proteins and so is exquisitely reliant on host factors for replication. However, we have minimal understanding of which cellular factors are involved. Discovering these will lead to better understanding of both the virus and host cell biology. BK polyomavirus can cause significant medical problems for kidney transplant patients, so the work may also identify new antiviral targets.

Recent publications:

Albecka A, Laine RF, Janssen AFJ, Kaminski CF, Crump CM (2016) HSV-1 glycoproteins are delivered to virus assembly sites through dynamin-dependent endocytosis. Traffic, 17(1):21-39

Guidelines for the use and interpretation of assays for monitoring autophagy. Klionsky DJ, Beale R and many others. Autophagy, 2016;12(1):1-222.

Evans GL, Caller LG, Foster V, and Crump CM. (2015) Anion homeostasis is important for non-lytic release of BK polyomavirus from infected cells. Open Biol. 5: 150041

Laine F, Albecka A, van de Linde S, Rees EJ, Crump CM*, Kaminski CF* (2015) Structural analysis of Herpes Simplex Virus by optical super-resolution imaging. Nat. Commun. 6:5980 doi: 10.1038/ncomms6980

A LC3-interacting motif in the influenza A virus M2 protein is required to subvert autophagy and maintain virion stability. Beale R, Wise H, Stuart A, Ravenhill BJ, Digard P, Randow F. Cell Host Microbe. 2014 Feb 12;15(2):239-47.